Bactericidal Permeability Increasing Protein Gene Variants in Children with Sepsis

Overview

Journal Intensive Care Med

Specialty Critical Care

Date 2007 Sep 28

PMID 17898994

Citations 18

Authors

Jaroslav Michalek

Petra Svetlikova

Michal Fedora

Michal Klimovic

Lenka Klapacova

Drahomira Bartosova

Lubomir Elbl

Hana Hrstkova

Jaroslav A Hubacek

Affiliations

Soon will be listed here.

Abstract

Objective: To evaluate the role of genetic polymorphisms of the bactericidal permeability increasing protein (BPI) in pediatric patients with sepsis.

Design: Prospective, single-center, case-control study at the pediatric intensive care unit (PICU) of a university hospital.

Patients: 345 consecutive pediatric patients admitted to the PICU with fever, systemic inflammatory response syndrome (SIRS), sepsis, severe sepsis, septic shock, or multiple organ distress syndrome (MODS).

Interventions: DNA was isolated and two BPI gene polymorphisms BPI (G545 > C) Taq and BPI (A645[ > G) 216 were studied in patients and compared with healthy controls.

Measurements And Results: Genetic analysis of the BPI Taq gene revealed significant differences between healthy controls and the subgroup of febrile patients (p = 0.0243), the subgroup of SIRS and sepsis (p = 0.0101), and the subgroup of severe sepsis, septic shock, and MODS (p = 0.0027), respectively. No statistically significant differences for the BPI 216 gene polymorphism were found between patient and healthy control groups. A statistically significant predisposition to Gram-negative sepsis in patients carrying the BPI Taq GG variant together with the BPI 216 AG or GG variant was revealed (p = 0.0081), and these haplotypes were also associated with death due to sepsis-related complications.

Conclusion: BPI Taq gene polymorphism is the accurate predictor of the severity of sepsis in children admitted to the PICU.

Citing Articles

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Wang X, Li R, Qian S, Yu D Pediatr Investig. 2023; 7(4):277-289.

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Association between innate immunity gene polymorphisms and neonatal sepsis development: a systematic review and meta-analysis.

Sljivancanin Jakovljevic T, Martic J, Jacimovic J, Nikolic N, Milasin J, Mitrovic T World J Pediatr. 2022; 18(10):654-670.

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A Polymorphism of Bactericidal/Permeability-Increasing Protein Affects Its Neutralization Efficiency towards Lipopolysaccharide.

Ederer K, Holzinger J, Maier K, Zeller L, Werner M, Toelge M Int J Mol Sci. 2022; 23(3).

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Association of Gene Polymorphism of Rs4358188, Rs2569190, Rs1143643 and Rs2664349 with Neonatal Sepsis.

Mustarim M, Yanwirasti Y, Jamsari J, Rukmono R, Nindrea R Open Access Maced J Med Sci. 2019; 7(17):2728-2733.

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Global Case-Fatality Rates in Pediatric Severe Sepsis and Septic Shock: A Systematic Review and Meta-analysis.

Tan B, Wong J, Sultana R, Koh J, Jit M, Mok Y JAMA Pediatr. 2019; 173(4):352-362.

PMID: 30742207 PMC: 6450287. DOI: 10.1001/jamapediatrics.2018.4839.

References

Andrews P, Azoulay E, Antonelli M, Brochard L, Brun-Buisson C, Dobb G . Year in review in intensive care medicine, 2004. III. Outcome, ICU organisation, scoring, quality of life, ethics, psychological problems and communication in the ICU, immunity and hemodynamics during sepsis, pediatric and neonatal critical care,.... Intensive Care Med. 2005; 31(3):356-72. DOI: 10.1007/s00134-005-2573-9. View

Rintala E, Peuravuori H, Pulkki K, Voipio-Pulkki L, Nevalainen T . Bactericidal/permeability-increasing protein (BPI) in sepsis correlates with the severity of sepsis and the outcome. Intensive Care Med. 2000; 26(9):1248-51. DOI: 10.1007/s001340000616. View

Alexander S, Bramson J, Foley R, Xing Z . Protection from endotoxemia by adenoviral-mediated gene transfer of human bactericidal/permeability-increasing protein. Blood. 2003; 103(1):93-9. DOI: 10.1182/blood-2003-02-0660. View

Ulevitch R . Recognition of bacterial endotoxins by receptor-dependent mechanisms. Adv Immunol. 1993; 53:267-89. DOI: 10.1016/s0065-2776(08)60502-7. View

Klein W, Tromm A, Folwaczny C, Hagedorn M, Duerig N, Epplen J . A polymorphism of the bactericidal/permeability increasing protein (BPI) gene is associated with Crohn's disease. J Clin Gastroenterol. 2005; 39(4):282-3. DOI: 10.1097/01.mcg.0000155127.20290.07. View

Dellinger R, Carlet J, Masur H, Gerlach H, Calandra T, Cohen J . Surviving Sepsis Campaign guidelines for management of severe sepsis and septic shock. Intensive Care Med. 2004; 30(4):536-55. DOI: 10.1007/s00134-004-2210-z. View

Levy M, Fink M, Marshall J, Abraham E, Angus D, Cook D . 2001 SCCM/ESICM/ACCP/ATS/SIS International Sepsis Definitions Conference. Crit Care Med. 2003; 31(4):1250-6. DOI: 10.1097/01.CCM.0000050454.01978.3B. View

Bindl L, Buderus S, Dahlem P, Demirakca S, Goldner M, Huth R . Gender-based differences in children with sepsis and ARDS: the ESPNIC ARDS Database Group. Intensive Care Med. 2003; 29(10):1770-3. DOI: 10.1007/s00134-003-1948-z. View

Weiss J . Bactericidal/permeability-increasing protein (BPI) and lipopolysaccharide-binding protein (LBP): structure, function and regulation in host defence against Gram-negative bacteria. Biochem Soc Trans. 2003; 31(Pt 4):785-90. DOI: 10.1042/bst0310785. View

10.

Leteurtre S, Martinot A, Duhamel A, Proulx F, Grandbastien B, Cotting J . Validation of the paediatric logistic organ dysfunction (PELOD) score: prospective, observational, multicentre study. Lancet. 2003; 362(9379):192-7. DOI: 10.1016/S0140-6736(03)13908-6. View

11.

Hubacek J, Pitha J, Skodova Z, Adamkova V, Podrapska I, Schmitz G . Polymorphisms in the lipopolysaccharide-binding protein and bactericidal/permeability-increasing protein in patients with myocardial infarction. Clin Chem Lab Med. 2003; 40(11):1097-100. DOI: 10.1515/CCLM.2002.191. View

12.

Ahrens P, Kattner E, Kohler B, Hartel C, Seidenberg J, Segerer H . Mutations of genes involved in the innate immune system as predictors of sepsis in very low birth weight infants. Pediatr Res. 2004; 55(4):652-6. DOI: 10.1203/01.PDR.0000112100.61253.85. View

13.

Miller S, Dykes D, Polesky H . A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res. 1988; 16(3):1215. PMC: 334765. DOI: 10.1093/nar/16.3.1215. View

14.

Watson R, Carcillo J, Linde-Zwirble W, Clermont G, Lidicker J, Angus D . The epidemiology of severe sepsis in children in the United States. Am J Respir Crit Care Med. 2002; 167(5):695-701. DOI: 10.1164/rccm.200207-682OC. View

15.

Pollack M, Ruttimann U, Getson P . Pediatric risk of mortality (PRISM) score. Crit Care Med. 1988; 16(11):1110-6. DOI: 10.1097/00003246-198811000-00006. View

16.

Pavcnik-Arnol M, Hojker S, Derganc M . Lipopolysaccharide-binding protein in critically ill neonates and children with suspected infection: comparison with procalcitonin, interleukin-6, and C-reactive protein. Intensive Care Med. 2004; 30(7):1454-60. DOI: 10.1007/s00134-004-2307-4. View

17.

Hamann L, Alexander C, Stamme C, Zahringer U, Schumann R . Acute-phase concentrations of lipopolysaccharide (LPS)-binding protein inhibit innate immune cell activation by different LPS chemotypes via different mechanisms. Infect Immun. 2004; 73(1):193-200. PMC: 538978. DOI: 10.1128/IAI.73.1.193-200.2005. View

18.

Wong H, Doughty L, Wedel N, White M, Nelson B, Havrilla N . Plasma bactericidal/permeability-increasing protein concentrations in critically ill children with the sepsis syndrome. Pediatr Infect Dis J. 1995; 14(12):1087-91. DOI: 10.1097/00006454-199512000-00011. View

19.

Froon A, Dentener M, Greve J, Ramsay G, Buurman W . Lipopolysaccharide toxicity-regulating proteins in bacteremia. J Infect Dis. 1995; 171(5):1250-7. DOI: 10.1093/infdis/171.5.1250. View

20.

Goldstein B, Giroir B, Randolph A . International pediatric sepsis consensus conference: definitions for sepsis and organ dysfunction in pediatrics. Pediatr Crit Care Med. 2005; 6(1):2-8. DOI: 10.1097/01.PCC.0000149131.72248.E6. View