Paradoxical DNA Repair and Peroxide Resistance Gene Conservation in Bacillus Pumilus SAFR-032

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2007 Sep 27

PMID 17895969

Citations 54

Authors

Jason Gioia

Shailaja Yerrapragada

Xiang Qin

Huaiyang Jiang

Okezie C Igboeli

Donna Muzny

Shannon Dugan-Rocha

Yan Ding

Alicia Hawes

Wen Liu

Lesette Perez

Christie Kovar

Huyen Dinh

Sandra Lee

Lynne Nazareth

Peter Blyth

Michael Holder

Christian Buhay

Madhan R Tirumalai

Yamei Liu

Indrani Dasgupta

Lina Bokhetache

Masaya Fujita

Fathi Karouia

Prahathees Eswara Moorthy

Johnathan Siefert

Akif Uzman

Prince Buzumbo

Avani Verma

Hiba Zwiya

Brian D McWilliams

Adeloa Olowu

Kenneth D Clinkenbeard

David Newcombe

Lisa Golebiewski

Joseph F Petrosino

Wayne L Nicholson

George E Fox

Kasthuri Venkateswaran

Sarah K Highlander

George M Weinstock

Affiliations

Soon will be listed here.

Abstract

Background: Bacillus spores are notoriously resistant to unfavorable conditions such as UV radiation, gamma-radiation, H2O2, desiccation, chemical disinfection, or starvation. Bacillus pumilus SAFR-032 survives standard decontamination procedures of the Jet Propulsion Lab spacecraft assembly facility, and both spores and vegetative cells of this strain exhibit elevated resistance to UV radiation and H2O2 compared to other Bacillus species.

Principal Findings: The genome of B. pumilus SAFR-032 was sequenced and annotated. Lists of genes relevant to DNA repair and the oxidative stress response were generated and compared to B. subtilis and B. licheniformis. Differences in conservation of genes, gene order, and protein sequences are highlighted because they potentially explain the extreme resistance phenotype of B. pumilus. The B. pumilus genome includes genes not found in B. subtilis or B. licheniformis and conserved genes with sequence divergence, but paradoxically lacks several genes that function in UV or H2O2 resistance in other Bacillus species.

Significance: This study identifies several candidate genes for further research into UV and H2O2 resistance. These findings will help explain the resistance of B. pumilus and are applicable to understanding sterilization survival strategies of microbes.

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