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Tamoxifen-inducible Cre-recombination in Articular Chondrocytes of Adult Col2a1-CreER(T2) Transgenic Mice

Overview
Journal Osteoarthritis Cartilage
Specialties Orthopedics
Rheumatology
Date 2007 Sep 25
PMID 17888690
Citations 39
Authors
M Zhu
M Chen
A C Lichtler
R J OKeefe
D Chen
Affiliations
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Abstract

Objective: To determine the specificity and efficiency of the tamoxifen (TM)-induced Cre-recombination in articular chondrocytes of adult Col2a1-CreER(T2) transgenic mice.

Methods: Col2a1-CreER(T2) transgenic mice were bred with Rosa26 reporter mice. Two-week-old Col2a1-CreER(T2);R26R mice were administered TM for 5 days and were sacrificed 1 and 6 months after TM induction. X-Gal staining was performed.

Results: Efficient Cre-recombination is achieved in adult articular chondrocytes 1 and 6 months after TM induction.

Conclusion: Our findings demonstrate that the Col2a1-CreER(T2) transgenic mouse model is a valuable tool to target genes specifically expressed in articular chondrocytes in a temporally controlled manner in adult mice.

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References
1.
Mao X, Fujiwara Y, Orkin S . Improved reporter strain for monitoring Cre recombinase-mediated DNA excisions in mice. Proc Natl Acad Sci U S A. 1999; 96(9):5037-42. PMC: 21812. DOI: 10.1073/pnas.96.9.5037. View
2.
Nakamura E, Nguyen M, Mackem S . Kinetics of tamoxifen-regulated Cre activity in mice using a cartilage-specific CreER(T) to assay temporal activity windows along the proximodistal limb skeleton. Dev Dyn. 2006; 235(9):2603-12. DOI: 10.1002/dvdy.20892. View
3.
Chen M, Lichtler A, Sheu T, Xie C, Zhang X, OKeefe R . Generation of a transgenic mouse model with chondrocyte-specific and tamoxifen-inducible expression of Cre recombinase. Genesis. 2007; 45(1):44-50. PMC: 2654410. DOI: 10.1002/dvg.20261. View
4.
Ovchinnikov D, Deng J, Ogunrinu G, Behringer R . Col2a1-directed expression of Cre recombinase in differentiating chondrocytes in transgenic mice. Genesis. 2000; 26(2):145-6. View
5.
Soriano P . Generalized lacZ expression with the ROSA26 Cre reporter strain. Nat Genet. 1999; 21(1):70-1. DOI: 10.1038/5007. View