Effects of Glucocorticoid on Adipocyte Size in Human Bone Marrow

Overview

Journal Med Mol Morphol

Publisher Springer

Specialties Cell Biology
Molecular Biology
Pathology

Date 2007 Sep 18

PMID 17874047

Citations 15

Authors

Masaru Kitajima

Masamori Shigematsu

Kenji Ogawa

Hajime Sugihara

Takao Hotokebuchi

Affiliations

Soon will be listed here.

Abstract

The pathogenesis of corticosteroid-induced femoral head necrosis is assumed to be related to lipid metabolism. Mature fat cells are believed to play a central role in lipid metabolism. The purpose of this study was to investigate the size of mature fat cells in the human femoral head after steroid treatment. Cancerous bone tissue was obtained from the femoral heads of 20 women who had undergone total hip arthroplasty. This bone tissue was subsequently incubated in a medium containing 10(-7) or 10(-5) M dexamethasone for 5 days. Mature fat cells from the bone marrow were observed by scanning electron microscopy, and the largest diameter of individual fat cells was measured. The size of the mature fat cells in human bone marrow increased after high-dose steroid treatment. The largest fat cell volume after steroid treatment was one and one-half times larger than that observed in the control. Steroid-induced osteonecrosis is known to sometimes occur after high-dose steroid treatment. These findings may indicate the pathogenetic factors in the early stage of steroid-induced osteonecrosis.

Citing Articles

Is core decompression and bone marrow concentrate with demineralized bone matrix and platelet-rich fibrin suitable for treating femoral head osteonecrosis?.

Cevolani L, Focaccia M, Spazzoli B, Bruschi A, Staals E, Dozza B J Hip Preserv Surg. 2025; 11(4):263-270.

PMID: 39839553 PMC: 11744473. DOI: 10.1093/jhps/hnae031.

Pathological mechanisms and related markers of steroid-induced osteonecrosis of the femoral head.

Li L, Zhao S, Leng Z, Chen S, Shi Y, Shi L Ann Med. 2024; 56(1):2416070.

PMID: 39529511 PMC: 11559024. DOI: 10.1080/07853890.2024.2416070.

[Analysis of incidence and clinical characteristics of osteonecrosis of femoral head in patients with systemic lupus erythematosus treated with glucocorticoid: A descriptive study based on a prospective cohort].

Xu Y, Chen S, Cai Q, Zhang C Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2023; 37(5):605-614.

PMID: 37190840 PMC: 10196977. DOI: 10.7507/1002-1892.202302026.

Lipid metabolism within the bone micro-environment is closely associated with bone metabolism in physiological and pathophysiological stages.

Wang B, Wang H, Li Y, Song L Lipids Health Dis. 2022; 21(1):5.

PMID: 34996476 PMC: 8742318. DOI: 10.1186/s12944-021-01615-5.

Changes of lipid and bone metabolism in broilers with spontaneous femoral head necrosis.

Liu K, Wang K, Wang L, Zhou Z Poult Sci. 2021; 100(3):100808.

PMID: 33518301 PMC: 7936160. DOI: 10.1016/j.psj.2020.10.062.

References

Drescher W, Weigert K, Bunger M, Ingerslev J, Bunger C, Hansen E . Femoral head blood flow reduction and hypercoagulability under 24 h megadose steroid treatment in pigs. J Orthop Res. 2004; 22(3):501-8. DOI: 10.1016/j.orthres.2003.10.002. View

Nishimura T, Matsumoto T, Nishino M, Tomita K . Histopathologic study of veins in steroid treated rabbits. Clin Orthop Relat Res. 1997; (334):37-42. View

Bjornheden T, Jakubowicz B, Levin M, Oden B, Eden S, Sjostrom L . Computerized determination of adipocyte size. Obes Res. 2004; 12(1):95-105. DOI: 10.1038/oby.2004.13. View

Mimura M, Tanaka N, Ichinose S, Kimijima Y, Amagasa T . Possible etiology of calculi formation in salivary glands: biophysical analysis of calculus. Med Mol Morphol. 2005; 38(3):189-95. DOI: 10.1007/s00795-005-0290-7. View

Motomura G, Yamamoto T, Miyanishi K, Yamashita A, Sueishi K, Iwamoto Y . Bone marrow fat-cell enlargement in early steroid-induced osteonecrosis--a histomorphometric study of autopsy cases. Pathol Res Pract. 2005; 200(11-12):807-11. DOI: 10.1016/j.prp.2004.10.003. View

Ichiseki T, Matsumoto T, Nishino M, Kaneuji A, Katsuda S . Oxidative stress and vascular permeability in steroid-induced osteonecrosis model. J Orthop Sci. 2004; 9(5):509-15. DOI: 10.1007/s00776-004-0816-1. View

Solomon L . Idiopathic necrosis of the femoral head: pathogenesis and treatment. Can J Surg. 1981; 24(6):573-8. View

Veenstra D, Best J, Hornberger J, Sullivan S, Hricik D . Incidence and long-term cost of steroid-related side effects after renal transplantation. Am J Kidney Dis. 1999; 33(5):829-39. DOI: 10.1016/s0272-6386(99)70414-2. View

Nakamura T, Matsumoto T, Nishino M, Tomita K, Kadoya M . Early magnetic resonance imaging and histologic findings in a model of femoral head necrosis. Clin Orthop Relat Res. 1997; (334):68-72. View

10.

Zalavras C, Dailiana Z, Elisaf M, Bairaktari E, Vlachogiannopoulos P, Katsaraki A . Potential aetiological factors concerning the development of osteonecrosis of the femoral head. Eur J Clin Invest. 2000; 30(3):215-21. DOI: 10.1046/j.1365-2362.2000.00621.x. View

11.

Pritchett J . Statin therapy decreases the risk of osteonecrosis in patients receiving steroids. Clin Orthop Relat Res. 2001; (386):173-8. DOI: 10.1097/00003086-200105000-00022. View

12.

JONES Jr J . Fat embolism, intravascular coagulation, and osteonecrosis. Clin Orthop Relat Res. 1993; (292):294-308. View

13.

Assouline-Dayan Y, Chang C, Greenspan A, Shoenfeld Y, Gershwin M . Pathogenesis and natural history of osteonecrosis. Semin Arthritis Rheum. 2002; 32(2):94-124. View

14.

Wang G, Sweet D, Reger S, Thompson R . Fat-cell changes as a mechanism of avascular necrosis of the femoral head in cortisone-treated rabbits. J Bone Joint Surg Am. 1977; 59(6):729-35. View

15.

Motomura G, Yamamoto T, Miyanishi K, Jingushi S, Iwamoto Y . Combined effects of an anticoagulant and a lipid-lowering agent on the prevention of steroid-induced osteonecrosis in rabbits. Arthritis Rheum. 2004; 50(10):3387-91. DOI: 10.1002/art.20517. View

16.

Rohatagi S, Barth J, Mollmann H, Hochhaus G, Soldner A, Mollmann C . Pharmacokinetics of methylprednisolone and prednisolone after single and multiple oral administration. J Clin Pharmacol. 1998; 37(10):916-25. DOI: 10.1002/j.1552-4604.1997.tb04266.x. View

17.

Taniguchi K, Matsuura K, Matsuoka T, Nakatani H, Nakano T, Furuya Y . A morphological study of the pacemaker cells of the aganglionic intestine in Hirschsprung's disease utilizing ls/ls model mice. Med Mol Morphol. 2005; 38(2):123-9. DOI: 10.1007/s00795-004-0283-y. View

18.

Wang G, Moga D, Richemer W, Sweet D, Reger S, Thompson R . Cortisone induced bone changes and its response to lipid clearing agents. Clin Orthop Relat Res. 1978; (130):81-5. View

19.

Sugihara H, Funatsumaru S, Yonemitsu N, Miyabara S, Toda S, Hikichi Y . A simple culture method of fat cells from mature fat tissue fragments. J Lipid Res. 1989; 30(12):1987-95. View

20.

Nishida S, Uchida H, Takeuchi M, Sui G, Mizutani S, Iwaki M . Scanning electron microscope study of the rabbit anterior chamber angle. Med Mol Morphol. 2005; 38(1):54-62. DOI: 10.1007/s00795-004-0274-z. View