RNA Interference of Metastasis-associated Gene 1 Inhibits Metastasis of B16F10 Melanoma Cells in a C57BL/6 Mouse Model

Overview

Journal Biol Cell

Specialty Cell Biology

Date 2007 Sep 18

PMID 17868030

Citations 16

Authors

Haili Qian

Jing Yu

Yunfeng Li

Haijuan Wang

Chongwen Song

Xueyan Zhang

Xiao Liang

Ming Fu

Chen Lin

Affiliations

Soon will be listed here.

Abstract

Background Information: MTA1 (metastasis-associated gene 1) has been reported to be overexpressed in cancers with high potential to metastasize. Studies of the molecular mechanisms revealed that MTA1 plays an important role in the process of metastasis of many types of cancer. However, the role of MTA1 in melanoma development is unclear.

Results: We have investigated the therapeutic value of MTA1 in the B16F10 melanoma cell line with the C57BL/6 mouse model. Studies in vitro showed that MTA1 promoted the metastatic ability of B16F10 cancer cells. MTA1 down-regulation by RNA interference greatly reversed the malignant phenotypes of cancer cells. Immunohistochemical staining of MTA1 in human melanoma samples confirmed the up-regulation of MTA1 in the process of carcinogenesis. Studies in vivo confirmed down-regulation of MTA1 suppressed the growth and experimental metastasis of B16F10 melanoma cells.

Conclusions: MTA1 plays an important role in melanoma development and metastasis. It has a promising potential as a target for in cancer gene therapy or chemotherapy.

Citing Articles

Clinical Significance and Oncogenic Activity of GRWD1 Overexpression in the Development of Colon Carcinoma.

Zhou X, Shang J, Liu X, Zhuang J, Yang Y, Zhang Y Onco Targets Ther. 2021; 14:1565-1580.

PMID: 33688204 PMC: 7936717. DOI: 10.2147/OTT.S290475.

Signatures of accelerated somatic evolution in gene promoters in multiple cancer types.

Smith K, Yadav V, Pedersen B, Shaknovich R, Geraci M, Pollard K Nucleic Acids Res. 2015; 43(11):5307-17.

PMID: 25934800 PMC: 4477653. DOI: 10.1093/nar/gkv419.

Coating Solid Lipid Nanoparticles with Hyaluronic Acid Enhances Antitumor Activity against Melanoma Stem-like Cells.

Shen H, Shi S, Zhang Z, Gong T, Sun X Theranostics. 2015; 5(7):755-71.

PMID: 25897340 PMC: 4402499. DOI: 10.7150/thno.10804.

YB-1 and MTA1 protein levels and not DNA or mRNA alterations predict for prostate cancer recurrence.

Sheridan C, Grogan T, Nguyen H, Galet C, Rettig M, Hsieh A Oncotarget. 2015; 6(10):7470-80.

PMID: 25797255 PMC: 4480693. DOI: 10.18632/oncotarget.3477.

Epstein-Barr virus-encoded latent membrane protein 2A promotes the epithelial-mesenchymal transition in nasopharyngeal carcinoma via metastatic tumor antigen 1 and mechanistic target of rapamycin signaling induction.

Lin Z, Wan X, Jiang R, Deng L, Gao Y, Tang J J Virol. 2014; 88(20):11872-85.

PMID: 25100829 PMC: 4178752. DOI: 10.1128/JVI.01867-14.