Identification, Expression and Tissue Distribution of a Renalase Homologue from Mouse

Overview

Journal Mol Biol Rep

Specialty Molecular Biology

Date 2007 Sep 12

PMID 17846919

Citations 9

Authors

Jian Wang

Shaoling Qi

Wei Cheng

Li Li

Fu Wang

Ying-Zi Li

Shu-Ping Zhang

Affiliations

Soon will be listed here.

Abstract

FAD (flavin adenine dinucleotide)-dependent monoamine oxidases play very important roles in many biological processes. A novel monoamine oxidase, named renalase, has been identified in human kidney recently and is found to be markedly reduced in patients with end-stage renal disease (ESRD). Here, we reported the identification of a renalase homologue from mouse, termed mMAO-C (mouse monoamine oxidase-C) after the monoamine oxidase-A and -B (MAO-A and -B). This gene locates on the mouse chromosome 19C1 and its coding region spans 7 exons. The deuced amino acid sequences were predicted to contain a typical secretive signal peptide and a conserved amine oxidase domain. Phylogenetic analysis and multiple sequences alignment indicated that mMAO-C-like sequences exist in all examined species and share significant similarities. This gene has been submitted to the NCBI GenBank database (Accession number: DQ788834). With expression vectors generated from the cloned mMAO-C gene, exogenous protein was effectively expressed in both prokaryotic and eukaryotic cells. Recombinant mMAO-C protein was secreted out of human cell lines, indicating the biological function of its signal peptide. Moreover, tissue expression pattern analysis revealed that mMAO-C gene is predominantly expressed in the mouse kidney and testicle, which implies that kidney and testicle are the main sources of renalase secretion. Shortly, this study provides an insight into understanding the physiological and biological functions of mMAO-C and its homologues in endocrine.

Citing Articles

Serum-to-urine renalase ratio and renalase fractional excretion in healthy adults and chronic kidney disease patients.

Serwin N, Wisniewska M, Cecerska-Heryc E, Safranow K, Skwirczynska E, Dolegowska B BMC Nephrol. 2020; 21(1):77.

PMID: 32131757 PMC: 7057639. DOI: 10.1186/s12882-020-01737-5.

Relationship between microRNA-146a expression and plasma renalase levels in hemodialyzed patients.

Dziedzic M, Powrozek T, Orlowska E, Koch W, Kukula-Koch W, Gawel K PLoS One. 2017; 12(6):e0179218.

PMID: 28614373 PMC: 5470705. DOI: 10.1371/journal.pone.0179218.

Establishing a low-expression renalase gene model in cardiac tissue of Sprague-Dawley rats.

Li X, Lin M, Xie Z, Huang R, Chen A, Jiang W Herz. 2015; 41(4):326-30.

PMID: 26612056 DOI: 10.1007/s00059-015-4370-8.

Normal values for urine renalase excretion in children.

Rybi-Szuminska A, Michaluk-Skutnik J, Osipiuk-Remza B, Kossakowska A, Wasilewska A Pediatr Nephrol. 2014; 29(11):2191-5.

PMID: 25060760 PMC: 4176974. DOI: 10.1007/s00467-014-2855-y.

Serum renalase is related to catecholamine levels and renal function.

Wang F, Li J, Xing T, Xie Y, Wang N Clin Exp Nephrol. 2014; 19(1):92-8.

PMID: 24590362 DOI: 10.1007/s10157-014-0951-8.

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