Hexameric Ring Structure of Human MCM10 DNA Replication Factor

Overview

Journal EMBO Rep

Specialty Molecular Biology

Date 2007 Sep 8

PMID 17823614

Citations 28

Authors

Andrei L Okorokov

Alastair Waugh

Julie Hodgkinson

Andal Murthy

Hye Kyung Hong

Elisabetta Leo

Michael B Sherman

Kai Stoeber

Elena V Orlova

Gareth H Williams

Affiliations

Soon will be listed here.

Abstract

The DNA replication factor minichromosome maintenance 10 (MCM10) is a conserved, abundant nuclear protein crucial for origin firing. During the transition from pre-replicative complexes to pre-initiation complexes, MCM10 recruitment to replication origins is required to provide a physical link between the MCM2-7 complex DNA helicase and DNA polymerases. Here, we report the molecular structure of human MCM10 as determined by electron microscopy and single-particle analysis. The MCM10 molecule is a ring-shaped hexamer with large central and smaller lateral channels and a system of inner chambers. This structure, together with biochemical data, suggests that this important protein uses its architecture to provide a docking module for assembly of the molecular machinery required for eukaryotic DNA replication.

Citing Articles

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Ouyang Y, Al-Amodi A, Tehseen M, Alhudhali L, Shirbini A, Takahashi M Nucleic Acids Res. 2024; 52(15):8880-8896.

PMID: 38967018 PMC: 11347169. DOI: 10.1093/nar/gkae565.

A critical threshold of MCM10 is required to maintain genome stability during differentiation of induced pluripotent stem cells into natural killer cells.

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PMID: 38262603 PMC: 10805602. DOI: 10.1098/rsob.230407.

The Mcm2-7-interacting domain of human mini-chromosome maintenance 10 (Mcm10) protein is important for stable chromatin association and origin firing.

Izumi M, Mizuno T, Yanagi K, Sugimura K, Okumura K, Imamoto N J Biol Chem. 2017; 292(31):13008-13021.

PMID: 28646110 PMC: 5546039. DOI: 10.1074/jbc.M117.779371.

An intact Mcm10 coiled-coil interaction surface is important for origin melting, helicase assembly and the recruitment of Pol-α to Mcm2-7.

Perez-Arnaiz P, Bruck I, Colbert M, Kaplan D Nucleic Acids Res. 2017; 45(12):7261-7275.

PMID: 28510759 PMC: 5499591. DOI: 10.1093/nar/gkx438.

Mcm10: A Dynamic Scaffold at Eukaryotic Replication Forks.

Baxley R, Bielinsky A Genes (Basel). 2017; 8(2).

PMID: 28218679 PMC: 5333062. DOI: 10.3390/genes8020073.

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