Tracing Shigatoxigenic Escherichia Coli O103, O145, and O174 Infections from Farm Residents to Cattle

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2007 Sep 7

PMID 17804658

Citations 2

Authors

Sirpa Heinikainen

Tarja Pohjanvirta

Marjut Eklund

Anja Siitonen

Sinikka Pelkonen

Affiliations

Soon will be listed here.

Abstract

Severe diarrheal infections caused by Shigatoxigenic Escherichia coli O103:H2:stx(1):eae-epsilon:ehx, O145:H28:stx(1):eae-gamma:ehx (two cases in a family), and O174:H21:stx(2c) in farm residents were traced to cattle. Molecular methods were applied to the isolation and characterization of the strains. The causative strains were also isolated from cattle samples 1 or 4 months later.

Citing Articles

Complete Genome Sequences of Two Escherichia coli O145:H28 Outbreak Strains of Food Origin.

Cooper K, Mandrell R, Louie J, Korlach J, Clark T, Parker C Genome Announc. 2014; 2(3).

PMID: 24855308 PMC: 4032123. DOI: 10.1128/genomeA.00482-14.

Agricultural, socioeconomic and environmental variables as risks for human verotoxigenic Escherichia coli (VTEC) infection in Finland.

Jalava K, Ollgren J, Eklund M, Siitonen A, Kuusi M BMC Infect Dis. 2011; 11:275.

PMID: 22008456 PMC: 3226588. DOI: 10.1186/1471-2334-11-275.

References

Blanco J, Blanco M, Alonso M, Mora A, Dahbi G, Coira M . Serotypes, virulence genes, and intimin types of Shiga toxin (verotoxin)-producing Escherichia coli isolates from human patients: prevalence in Lugo, Spain, from 1992 through 1999. J Clin Microbiol. 2004; 42(1):311-9. PMC: 321739. DOI: 10.1128/JCM.42.1.311-319.2004. View

Fratamico P, Briggs C, Needle D, Chen C, DebRoy C . Sequence of the Escherichia coli O121 O-antigen gene cluster and detection of enterohemorrhagic E. coli O121 by PCR amplification of the wzx and wzy genes. J Clin Microbiol. 2003; 41(7):3379-83. PMC: 165269. DOI: 10.1128/JCM.41.7.3379-3383.2003. View

Pearce M, Jenkins C, Vali L, Smith A, Knight H, Cheasty T . Temporal shedding patterns and virulence factors of Escherichia coli serogroups O26, O103, O111, O145, and O157 in a cohort of beef calves and their dams. Appl Environ Microbiol. 2004; 70(3):1708-16. PMC: 368277. DOI: 10.1128/AEM.70.3.1708-1716.2004. View

Perelle S, Dilasser F, Grout J, Fach P . Detection by 5'-nuclease PCR of Shiga-toxin producing Escherichia coli O26, O55, O91, O103, O111, O113, O145 and O157:H7, associated with the world's most frequent clinical cases. Mol Cell Probes. 2004; 18(3):185-92. DOI: 10.1016/j.mcp.2003.12.004. View

De Baets L, Van der Taelen I, De Filette M, Pierard D, Allison L, De Greve H . Genetic typing of shiga toxin 2 variants of Escherichia coli by PCR-restriction fragment length polymorphism analysis. Appl Environ Microbiol. 2004; 70(10):6309-14. PMC: 522131. DOI: 10.1128/AEM.70.10.6309-6314.2004. View

Adu-Bobie J, Frankel G, Bain C, Goncalves A, Trabulsi L, Douce G . Detection of intimins alpha, beta, gamma, and delta, four intimin derivatives expressed by attaching and effacing microbial pathogens. J Clin Microbiol. 1998; 36(3):662-8. PMC: 104605. DOI: 10.1128/JCM.36.3.662-668.1998. View

Bastian S, Carle I, Grimont F . Comparison of 14 PCR systems for the detection and subtyping of stx genes in Shiga-toxin-producing Escherichia coli. Res Microbiol. 1998; 149(7):457-72. DOI: 10.1016/s0923-2508(98)80001-6. View

Keskimaki M, Saari M, Heiskanen T, Siitonen A . Shiga toxin-producing Escherichia coli in finland from 1990 through 1997: prevalence and characteristics of isolates. J Clin Microbiol. 1998; 36(12):3641-6. PMC: 105255. DOI: 10.1128/JCM.36.12.3641-3646.1998. View

Prager R, Annemuller S, Tschape H . Diversity of virulence patterns among shiga toxin-producing Escherichia coli from human clinical cases-need for more detailed diagnostics. Int J Med Microbiol. 2005; 295(1):29-38. DOI: 10.1016/j.ijmm.2004.12.009. View

10.

Blanco M, Schumacher S, Tasara T, Zweifel C, Blanco J, Dahbi G . Serotypes, intimin variants and other virulence factors of eae positive Escherichia coli strains isolated from healthy cattle in Switzerland. Identification of a new intimin variant gene (eae-eta2). BMC Microbiol. 2005; 5:23. PMC: 1142320. DOI: 10.1186/1471-2180-5-23. View

11.

Gerner-Smidt P, Kincaid J, Kubota K, Hise K, Hunter S, Fair M . Molecular surveillance of shiga toxigenic Escherichia coli O157 by PulseNet USA. J Food Prot. 2005; 68(9):1926-31. DOI: 10.4315/0362-028x-68.9.1926. View

12.

Brooks J, Sowers E, Wells J, Greene K, Griffin P, Hoekstra R . Non-O157 Shiga toxin-producing Escherichia coli infections in the United States, 1983-2002. J Infect Dis. 2005; 192(8):1422-9. DOI: 10.1086/466536. View

13.

Eklund M, Nuorti J, Ruutu P, Siitonen A . Shigatoxigenic Escherichia coli (STEC) infections in Finland during 1998-2002: a population-based surveillance study. Epidemiol Infect. 2005; 133(5):845-52. PMC: 2870315. DOI: 10.1017/S0950268805004450. View

14.

Beutin L, Kong Q, Feng L, Wang Q, Krause G, Leomil L . Development of PCR assays targeting the genes involved in synthesis and assembly of the new Escherichia coli O 174 and O 177 O antigens. J Clin Microbiol. 2005; 43(10):5143-9. PMC: 1248525. DOI: 10.1128/JCM.43.10.5143-5149.2005. View

15.

Naylor S, Gally D, Low J . Enterohaemorrhagic E. coli in veterinary medicine. Int J Med Microbiol. 2005; 295(6-7):419-41. DOI: 10.1016/j.ijmm.2005.07.010. View

16.

Barrett T, Gerner-Smidt P, Swaminathan B . Interpretation of pulsed-field gel electrophoresis patterns in foodborne disease investigations and surveillance. Foodborne Pathog Dis. 2006; 3(1):20-31. DOI: 10.1089/fpd.2006.3.20. View

17.

Ribot E, Fair M, Gautom R, Cameron D, Hunter S, Swaminathan B . Standardization of pulsed-field gel electrophoresis protocols for the subtyping of Escherichia coli O157:H7, Salmonella, and Shigella for PulseNet. Foodborne Pathog Dis. 2006; 3(1):59-67. DOI: 10.1089/fpd.2006.3.59. View

18.

Eklund M, Bielaszewska M, Nakari U, Karch H, Siitonen A . Molecular and phenotypic profiling of sorbitol-fermenting Escherichia coli O157:H- human isolates from Finland. Clin Microbiol Infect. 2006; 12(7):634-41. DOI: 10.1111/j.1469-0691.2006.01478.x. View

19.

Beutin L, Bulte M, Weber A, Zimmermann S, Gleier K . Investigation of human infections with verocytotoxin-producing strains of Escherichia coli (VTEC) belonging to serogroup O118 with evidence for zoonotic transmission. Epidemiol Infect. 2000; 125(1):47-54. PMC: 2869568. DOI: 10.1017/s0950268899004094. View

20.

Pradel N, Boukhors K, Bertin Y, Forestier C, Martin C, Livrelli V . Heterogeneity of Shiga toxin-producing Escherichia coli strains isolated from hemolytic-uremic syndrome patients, cattle, and food samples in central France. Appl Environ Microbiol. 2001; 67(6):2460-8. PMC: 92895. DOI: 10.1128/AEM.67.6.2460-2468.2001. View