Affinity Thresholds for Membrane Fusion Triggering by Viral Glycoproteins

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2007 Sep 7

PMID 17804513

Citations 47

Authors

Kosei Hasegawa

Chunling Hu

Takafumi Nakamura

James D Marks

Stephen J Russell

Kah-Whye Peng

Affiliations

Soon will be listed here.

Abstract

Enveloped viruses trigger membrane fusion to gain entry into cells. The receptor affinities of their attachment proteins vary greatly, from 10(-4) M to 10(-9) M, but the significance of this is unknown. Using six retargeted measles viruses that bind to Her-2/neu with a 5-log range in affinity, we show that receptor affinity has little impact on viral attachment but is nevertheless a key determinant of infectivity and intercellular fusion. For a given cell surface receptor density, there is an affinity threshold above which cell-cell fusion proceeds efficiently. Suprathreshold affinities do not further enhance the efficiency of membrane fusion.

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