Increased Calpain Correlates with Th1 Cytokine Profile in PBMCs from MS Patients

Overview

Journal J Neuroimmunol

Specialty Neurology

Date 2007 Sep 4

PMID 17765980

Citations 32

Authors

Sarah A Imam

Mary K Guyton

Azizul Haque

Arthur Vandenbark

William R Tyor

Swapan K Ray

Naren L Banik

Affiliations

Soon will be listed here.

Abstract

Multiple sclerosis (MS) is a devastating autoimmune demyelinating disease of the central nervous system (CNS). This study investigated whether expression and activity of the calcium-activated protease calpain correlated with Th1/Th2 dysregulation in MS patients during states of relapse and remission. Calpain expression and activity were significantly increased in peripheral blood mononuclear cells (PBMCs) from MS patients, compared to controls, with the highest expression and activity noted during relapse. Th1 cytokines were highest and Th2 cytokines were lowest in MS patients during relapse. Treatment with calpain inhibitor, calpeptin, decreased Th1 cytokines in PBMCs from MS patients. Calpain inhibitor also reduced degradation of myelin basic protein (MBP) by inhibiting the calpain secreted from MBP-specific T cells. Taken together, these results suggested calpain involvement in Th1/Th2 dysregulation in MS patients.

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