Genome-wide Genetic Aberrations of Thymoma Using CDNA Microarray Based Comparative Genomic Hybridization

Overview

Journal BMC Genomics

Publisher Biomed Central

Specialty Genetics

Date 2007 Sep 4

PMID 17764580

Citations 16

Authors

Gui Youn Lee

Woo Ick Yang

Hei Cheul Jeung

Sang Chul Kim

Min Young Seo

Chan Hee Park

Hyun Cheol Chung

Sun Young Rha

Affiliations

Soon will be listed here.

Abstract

Background: Thymoma is a heterogeneous group of tumors in biology and clinical behavior. Even though thymoma is divided into five subgroups following the World Health Organization classification, the nature of the disease is mixed within the subgroups.

Results: We investigated the molecular characteristics of genetic changes variation of thymoma using cDNA microarray based-comparative genomic hybridization (CGH) with a 17 K cDNA microarray in an indirect, sex-matched design. Genomic DNA from the paraffin embedded 39 thymoma tissues (A 6, AB 11, B1 7, B2 7, B3 8) labeled with Cy-3 was co-hybridized with the reference placenta gDNA labeled with Cy-5. Using the CAMVS software, we investigated the deletions on chromosomes 1, 2, 3, 4, 5, 6, 8, 12, 13 and 18 throughout the thymoma. Then, we evaluated the genetic variations of thymoma based on the subgroups and the clinical behavior. First, the 36 significant genes differentiating five subgroups were selected by Significance Analysis of Microarray. Based on these genes, type AB was suggested to be heterogeneous at the molecular level as well as histologically. Next, we observed that the thymoma was divided into A, B (1, 2) and B3 subgroups with 33 significant genes. In addition, we selected 70 genes differentiating types A and B3, which differ largely in clinical behaviors. Finally, the 11 heterogeneous AB subtypes were able to correctly assign into A and B (1, 2) types based on their genetic characteristics.

Conclusion: In our study, we observed the genome-wide chromosomal aberrations of thymoma and identified significant gene sets with genetic variations related to thymoma subgroups, which might provide useful information for thymoma pathobiology.

Citing Articles

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The Molecular Landscape of Thymic Epithelial Tumors: A Comprehensive Review.

Elm L, Levidou G Int J Mol Sci. 2024; 25(3).

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Kim D, Lim Y, Kim S, Ock C, Youk J, Kim M Thorac Cancer. 2023; 14(30):3001-3011.

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The immune landscape of human thymic epithelial tumors.

Xin Z, Lin M, Hao Z, Chen D, Chen Y, Chen X Nat Commun. 2022; 13(1):5463.

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Using proteomic profiling to characterize protein signatures of different thymoma subtypes.

Lai L, Sun Q, Chen Y, Hsiao Y, Lu T, Tsai M BMC Cancer. 2019; 19(1):796.

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