DNA Ejection from Bacteriophage: Towards a General Behavior for Osmotic-suppression Experiments

Overview

Journal Eur Phys J E Soft Matter

Publisher EDP Sciences

Specialty Biophysics

Date 2007 Sep 1

PMID 17762912

Citations 11

Authors

M Castelnovo

A Evilevitch

Affiliations

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Abstract

We present in this work in vitro measurements of the force ejecting DNA from two distinct bacteriophages (T5 and lambda using the osmotic-suppression technique. Our data are analyzed by revisiting the current theories of DNA packaging in spherical capsids. In particular we show that a simplified analytical model based on bending considerations only is able to account quantitatively for the experimental findings. Physical and biological consequences are discussed.

Citing Articles

Temperature-induced DNA density transition in phage λ capsid revealed with contrast-matching SANS.

Villanueva Valencia J, Tsimtsirakis E, Krueger S, Evilevitch A Proc Natl Acad Sci U S A. 2023; 120(45):e2220518120.

PMID: 37903276 PMC: 10636372. DOI: 10.1073/pnas.2220518120.

'SAXS-osmometer' method provides measurement of DNA pressure in viral capsids and delivers an empirical equation of state.

Villanueva Valencia J, Li D, Casjens S, Evilevitch A Nucleic Acids Res. 2023; 51(21):11415-11427.

PMID: 37889048 PMC: 10681747. DOI: 10.1093/nar/gkad852.

The mobility of packaged phage genome controls ejection dynamics.

Evilevitch A Elife. 2018; 7.

PMID: 30178745 PMC: 6122950. DOI: 10.7554/eLife.37345.

Translocation of a granular chain in a horizontally vibrated saw-tooth channel.

Mortazavi F, Habibi M, Oskoee E Eur Phys J E Soft Matter. 2016; 39(10):93.

PMID: 27761780 DOI: 10.1140/epje/i2016-16093-6.

Bacteriophage P22 ejects all of its internal proteins before its genome.

Jin Y, Sdao S, Dover J, Porcek N, Knobler C, Gelbart W Virology. 2015; 485:128-34.

PMID: 26245366 PMC: 4619139. DOI: 10.1016/j.virol.2015.07.006.

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