Rapid Flow Cytometric Analysis of the Cell Cycle in Intact Plant Tissues

Overview

Journal Science

Specialty Science

Date 1983 Jun 3

PMID 17754551

Citations 484

Authors

D W Galbraith

K R Harkins

J M Maddox

N M Ayres

D P Sharma

E Firoozabady

Affiliations

Soon will be listed here.

Abstract

Mechanical chopping of plant tissues in the presence of mithramycin released intact nuclei representative of the cells within the tissues. The amount of nuclear DNA in the homogenates of monocotyledonous and dicotyledonous plants was accurately and rapidly determined by flow microfluorometry, and the distribution of nuclei involved in the cell cycle was charted for tissues selected from different physical locations or developmental stages.

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