110-kilodalton Recombinant Protein Which is Immunoreactive with Sera from Humans, Dogs, and Horses with Lyme Borreliosis

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1991 Nov 1

PMID 1774245

Citations 4

Authors

A C Caputa

M P Murtaugh

R F Bey

K I Loken

Affiliations

Soon will be listed here.

Abstract

EcoRI-digested DNA from Borrelia burgdorferi was ligated into the dephosphorylated vector pWR590 and transformed into Escherichia coli DH5 alpha. When the gene library was screened, 20 clones reacted with pooled dog sera with high titers (immunofluorescent antibody titer, greater than or equal to 1,280) to this spirochete. One clone expressed a 110-kDa antigen that reacted strongly with the high-titered pooled sera from dogs with Lyme borreliosis and serum from goats immunized with B. burgdorferi. The 110-kDa protein was serum from goats immunized with B. burgdorferi. The 110-kDa protein was expressed with and without isopropyl-beta-D-thiogalactosidase, indicating the protein is not a fusion protein with beta-galactosidase. Monospecific antisera to the 110-kDa antigen recognized a 75-kDa Borrelia protein. Of the sera that reacted with B. burgdorferi by immunoblotting; 57, 100, and 83% of human, dog, and horse serum samples, respectively, reacted with the 110-kDa protein. Sera from individuals that tested negative with a B. burgdorferi lysate with immunoblotting showed no reaction with the 110-kDa protein. The 110-kDa antigen appears to be useful for the diagnosis of Lyme borreliosis.

Citing Articles

Assessing knowledge, attitudes, and practices of Canadian veterinarians with regard to Lyme disease in dogs.

Nichol G, Weese J, Evason M, Clow K J Vet Intern Med. 2021; 35(1):294-302.

PMID: 33421198 PMC: 7848372. DOI: 10.1111/jvim.16022.

Protection of C3H/He mice from experimental Borrelia burgdorferi infection by immunization with a 110-kilodalton fusion protein.

Bey R, Larson M, Lowery D, Lee B, Knutson K, Simonson R Infect Immun. 1995; 63(8):3213-17.

PMID: 7622251 PMC: 173440. DOI: 10.1128/iai.63.8.3213-3217.1995.

Evaluation of genetic divergence among Borrelia burgdorferi isolates by use of OspA, fla, HSP60, and HSP70 gene probes.

Wallich R, Helmes C, Schaible U, Lobet Y, Moter S, Kramer M Infect Immun. 1992; 60(11):4856-66.

PMID: 1356932 PMC: 258241. DOI: 10.1128/iai.60.11.4856-4866.1992.

Comparison of whole-cell antibodies and an antigenic flagellar epitope of Borrelia burgdorferi in serologic tests for diagnosis of Lyme borreliosis.

Magnarelli L, Fikrig E, Berland R, Anderson J, Flavell R J Clin Microbiol. 1992; 30(12):3158-62.

PMID: 1280650 PMC: 270607. DOI: 10.1128/jcm.30.12.3158-3162.1992.

References

Lawson J, Steere A . Lyme arthritis: radiologic findings. Radiology. 1985; 154(1):37-43. DOI: 10.1148/radiology.154.1.3964949. View

Magnarelli L, Miller J, Anderson J, Riviere G . Cross-reactivity of nonspecific treponemal antibody in serologic tests for Lyme disease. J Clin Microbiol. 1990; 28(6):1276-9. PMC: 267918. DOI: 10.1128/jcm.28.6.1276-1279.1990. View

Wallich R, Moter S, Simon M, Ebnet K, Heiberger A, Kramer M . The Borrelia burgdorferi flagellum-associated 41-kilodalton antigen (flagellin): molecular cloning, expression, and amplification of the gene. Infect Immun. 1990; 58(6):1711-9. PMC: 258713. DOI: 10.1128/iai.58.6.1711-1719.1990. View

Steere A, Brinckerhoff C, Miller D, Drinker H, Harris Jr E, Malawista S . Elevated levels of collagenase and prostaglandin E2 from synovium associated with erosion of cartilage and bone in a patient with chronic Lyme arthritis. Arthritis Rheum. 1980; 23(5):591-9. DOI: 10.1002/art.1780230511. View

Young R, Davis R . Efficient isolation of genes by using antibody probes. Proc Natl Acad Sci U S A. 1983; 80(5):1194-8. PMC: 393560. DOI: 10.1073/pnas.80.5.1194. View

Russell H, Sampson J, Schmid G, WILKINSON H, Plikaytis B . Enzyme-linked immunosorbent assay and indirect immunofluorescence assay for Lyme disease. J Infect Dis. 1984; 149(3):465-70. DOI: 10.1093/infdis/149.3.465. View

Barbour A, BURGDORFER W, GRUNWALDT E, Steere A . Antibodies of patients with Lyme disease to components of the Ixodes dammini spirochete. J Clin Invest. 1983; 72(2):504-15. PMC: 1129208. DOI: 10.1172/jci110998. View

Lissman B, Bosler E, Camay H, Ormiston B, Benach J . Spirochete-associated arthritis (Lyme disease) in a dog. J Am Vet Med Assoc. 1984; 185(2):219-20. View

BURGDORFER W, Barbour A, Hayes S, Benach J, GRUNWALDT E, Davis J . Lyme disease-a tick-borne spirochetosis?. Science. 1982; 216(4552):1317-9. DOI: 10.1126/science.7043737. View

10.

Steere A, Batsford W, Weinberg M, Alexander J, Berger H, Wolfson S . Lyme carditis: cardiac abnormalities of Lyme disease. Ann Intern Med. 1980; 93(1):8-16. DOI: 10.7326/0003-4819-93-1-8. View

11.

Reik L, Steere A, Bartenhagen N, Shope R, Malawista S . Neurologic abnormalities of Lyme disease. Medicine (Baltimore). 1979; 58(4):281-94. DOI: 10.1097/00005792-197907000-00001. View

12.

Steere A, Gibofsky A, Patarroyo M, Winchester R, Hardin J, Malawista S . Chronic Lyme arthritis. Clinical and immunogenetic differentiation from rheumatoid arthritis. Ann Intern Med. 1979; 90(6):896-901. DOI: 10.7326/0003-4819-90-6-896. View

13.

Dryer R, Goellner P, Carney A . Lyme arthritis in Wisconsin. JAMA. 1979; 241(5):498-9. View

14.

Bey R, Johnson R . Protein-free and low-protein media for the cultivation of Leptospira. Infect Immun. 1978; 19(2):562-9. PMC: 414120. DOI: 10.1128/iai.19.2.562-569.1978. View

15.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

16.

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

17.

Fikrig E, Barthold S, Kantor F, Flavell R . Protection of mice against the Lyme disease agent by immunizing with recombinant OspA. Science. 1990; 250(4980):553-6. DOI: 10.1126/science.2237407. View

18.

Collins C, Peltz G . Immunoreactive epitopes on an expressed recombinant flagellar protein of Borrelia burgdorferi. Infect Immun. 1991; 59(2):514-20. PMC: 257779. DOI: 10.1128/iai.59.2.514-520.1991. View

19.

Pachner A, Steere A . The triad of neurologic manifestations of Lyme disease: meningitis, cranial neuritis, and radiculoneuritis. Neurology. 1985; 35(1):47-53. DOI: 10.1212/wnl.35.1.47. View

20.

Howe T, Laquier F, Barbour A . Organization of genes encoding two outer membrane proteins of the Lyme disease agent Borrelia burgdorferi within a single transcriptional unit. Infect Immun. 1986; 54(1):207-12. PMC: 260138. DOI: 10.1128/iai.54.1.207-212.1986. View