Nucleation Time of Gall Bladder Bile in Gall Stone Patients: Influence of Bile Acid Treatment

Overview

Journal Gut

Specialty Gastroenterology

Date 1991 Dec 1

PMID 1773966

Citations 3

Authors

S Sahlin

J Ahlberg

B Angelin

E Reihner

K Einarsson

Affiliations

Soon will be listed here.

Abstract

The time required for precipitation of cholesterol crystals (nucleation time, NT) was determined and related to the cholesterol saturation in gall bladder bile of gall stone free subjects (n = 11), patients with pigment stones (n = 3), and patients with cholesterol gall stones (n = 30) undergoing cholecystectomy. Seven of the gall stone patients had been treated with chenodeoxycholic acid (CDCA) and nine with ursodeoxycholic acid (UDCA), 15 mg/kg/day for three weeks before operation. NT was longer in gall stone free subjects (mean, 20 days), patients with pigment stones (14 days) and patients treated with CDCA (24 days) and UDCA (17 days) compared with untreated patients with cholesterol gall stones (1.5 days). In spite of low cholesterol saturation and prolonged NT, and in contrast to those treated with CDCA, four of the nine patients treated with UDCA had cholesterol crystals in their bile. These observations give further support to the concept that the mechanism for inducing gall stone dissolution may be different for CDCA and UDCA.

Citing Articles

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References

HOLZBACH R . Recent progress in understanding cholesterol crystal nucleation as a precursor to human gallstone formation. Hepatology. 1986; 6(6):1403-6. DOI: 10.1002/hep.1840060630. View

Tint G, Salen G, SHEFER S . Effect of ursodeoxycholic acid and chenodeoxycholic acid on cholesterol and bile acid metabolism. Gastroenterology. 1986; 91(4):1007-18. DOI: 10.1016/0016-5085(86)90708-0. View

Nilsell K, Angelin B, Liljeqvist L, Einarsson K . Biliary lipid output and bile acid kinetics in cholesterol gallstone disease. Evidence for an increased hepatic secretion of cholesterol in Swedish patients. Gastroenterology. 1985; 89(2):287-93. DOI: 10.1016/0016-5085(85)90328-2. View

SALVIOLI G, Igimi H, Carey M . Cholesterol gallstone dissolution in bile. Dissolution kinetics of crystalline cholesterol monohydrate by conjugated chenodeoxycholate-lecithin and conjugated ursodeoxycholate-lecithin mixtures: dissimilar phase equilibria and dissolution mechanisms. J Lipid Res. 1983; 24(6):701-20. View

Lee S, Lamont J, Carey M . Role of gallbladder mucus hypersecretion in the evolution of cholesterol gallstones. J Clin Invest. 1981; 67(6):1712-23. PMC: 370748. DOI: 10.1172/jci110209. View

Corrigan O, Su C, Higuchi W, Hofmann A . Mesophase formation during cholesterol dissolution in ursodeoxycholate-lecithin solutions: new mechanism for gallstone dissolution in humans. J Pharm Sci. 1980; 69(7):869-71. DOI: 10.1002/jps.2600690739. View

Ahlberg J, Angelin B, Einarsson K, Hellstrom K, Leijd B . Biliary lipid composition in normo- and hyperlipoproteinemia. Gastroenterology. 1980; 79(1):90-4. View

Angelin B, Einarsson K, Leijd B . Biliary lipid composition during treatment with different hypolipidaemic drugs. Eur J Clin Invest. 1979; 9(3):185-90. DOI: 10.1111/j.1365-2362.1979.tb00921.x. View

Lee S, Lim T, Scott A . Carbohydrate moieties of glycoproteins in human hepatic and gall-bladder bile, gall-bladder mucosa and gall stones. Clin Sci (Lond). 1979; 56(6):533-8. DOI: 10.1042/cs0560533. View

10.

Holan K, HOLZBACH R, Hermann R, Cooperman A, Claffey W . Nucleation time: a key factor in the pathogenesis of cholesterol gallstone disease. Gastroenterology. 1979; 77(4 Pt 1):611-7. View

11.

Carey M . Critical tables for calculating the cholesterol saturation of native bile. J Lipid Res. 1978; 19(8):945-55. View

12.

Wahlin T . Effects of lithogenic diets on mouse gallbladder epithelium. A histochemical, cytochemical and morphometric study. Virchows Arch B Cell Pathol. 1976; 22(4):273-86. DOI: 10.1007/BF02889221. View

13.

Bell G, Whitney B, Dowling R . Gallstone dissolution in man using chenodeoxycholic acid. Lancet. 1972; 2(7789):1213-6. DOI: 10.1016/s0140-6736(72)92266-0. View

14.

Fausa O, SKALHEGG B . Quantitative determination of bile acids and their conjugates using thin-layer chromatography and a purified 3alpha-hydroxysteroid dehydrogenase. Scand J Gastroenterol. 1974; 9(3):249-54. View

15.

Trotman B, Ostrow J, Soloway R . Pigment vs cholesterol cholelithiasis: comparison of stone and bile composition. Am J Dig Dis. 1974; 19(7):585-90. DOI: 10.1007/BF01073011. View

16.

HOLZBACH R, Marsh M, Olszewski M, Holan K . Cholesterol solubility in bile. Evidence that supersaturated bile is frequent in healthy man. J Clin Invest. 1973; 52(6):1467-79. PMC: 302412. DOI: 10.1172/JCI107321. View

17.

Danzinger R, Hofmann A, SCHOENFIELD L, Thistle J . Dissolution of cholesterol gallstones by chenodeoxycholic acid. N Engl J Med. 1972; 286(1):1-8. DOI: 10.1056/NEJM197201062860101. View

18.

Roda A, Festi D, Sama C, Mazzella G, Alini R, Roda E . Enzymatic determination of cholesterol in bile. Clin Chim Acta. 1975; 64(3):337-41. DOI: 10.1016/0009-8981(75)90364-2. View

19.

Rouser G, Fkeischer S, Yamamoto A . Two dimensional then layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots. Lipids. 1970; 5(5):494-6. DOI: 10.1007/BF02531316. View

20.

Bouchier I, COOPERBAND S . Mucous substances and viscosity of normal and pathological human bile. Gastroenterology. 1965; 49(4):343-53. View