Interaction of an Amphipathic Peptide with Phosphatidycholine/phosphatidylethanolamine Mixed Membranes

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 2007 Aug 21

PMID 17704174

Citations 10

Authors

Keisuke Shintou

Minoru Nakano

Tomoari Kamo

Yoshihiro Kuroda

Tetsurou Handa

Affiliations

Soon will be listed here.

Abstract

The effect of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) in mixed membranes with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) on interaction with a class A amphipathic peptide, Ac-DWLKAFYDKVAEKLKEAF-NH(2) (Ac-18A-NH(2)), was investigated. The fluorescence lifetime of 2-(9-anthroyloxy)stearic acid and (2)H NMR spectra were used to evaluate the penetration of water molecules into the membrane interface and the order of lipid acyl chains, respectively. The results demonstrated that DOPE in the mixed membranes decreased the fluorescence lifetime and increased the acyl-chain order, and that Ac-18A-NH(2) affected them more for membranes with higher DOPE fractions. The partition coefficient (K(p)) of the peptide to the mixed membranes was increased with the increase in the DOPE mole fractions. From the temperature dependence of the K(p) values, the binding of Ac-18A-NH(2) to POPC/DOPE mixed membranes was found to be entropy-driven. The formation of an alpha-helix at the membrane's surface is supposed to induce positive curvature strain, which decreases the headgroup hydration and acyl-chain order of lipids. Thus, the binding of Ac-18A-NH(2) to membranes is entropically more favorable at higher DOPE fractions since the peptide's insertion into the membrane can decrease the order parameter and unfavorable headgroup hydration, which explains the enhanced peptide binding.

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