Transcriptomic Analysis of the Sulfate Starvation Response of Pseudomonas Aeruginosa

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2007 Aug 7

PMID 17675390

Citations 43

Authors

Tewes Tralau

Stephane Vuilleumier

Christelle Thibault

Barry J Campbell

C Anthony Hart

Michael A Kertesz

Affiliations

Soon will be listed here.

Abstract

Pseudomonas aeruginosa is an opportunistic pathogen that causes a number of infections in humans, but is best known for its association with cystic fibrosis. It is able to use a wide range of sulfur compounds as sources of sulfur for growth. Gene expression in response to changes in sulfur supply was studied in P. aeruginosa E601, a cystic fibrosis isolate that displays mucin sulfatase activity, and in P. aeruginosa PAO1. A large family of genes was found to be upregulated by sulfate limitation in both isolates, encoding sulfatases and sulfonatases, transport systems, oxidative stress proteins, and a sulfate-regulated TonB/ExbBD complex. These genes were localized in five distinct islands on the genome and encoded proteins with a significantly reduced content of cysteine and methionine. Growth of P. aeruginosa E601 with mucin as the sulfur source led not only to a sulfate starvation response but also to induction of genes involved with type III secretion systems.

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