Interaction of L-arginine-methyl Ester and Sonic Hedgehog in Liver Ischemia-reperfusion Injury in the Rats

Overview

Journal World J Gastroenterol

Publisher Baishideng Publishing Group

Specialty Gastroenterology

Date 2007 Jul 28

PMID 17657839

Citations 6

Authors

Mehmet-Cudi Tuncer

Hayrettin Ozturk

Huseyin Buyukbayram

Hulya Ozturk

Affiliations

Soon will be listed here.

Abstract

Aim: To investigate the role of Sonic hedgehog (Shh) on the course of liver ischemia and reperfusion (I/R) in rats, and the interaction between treatment with nitric oxide donor L-Arginine-methyl ester (L-Arg) and up-regulation of Shh expression.

Methods: A total of 30 male Sprague-Dawley rats weighing 220-240 g were used in this study. Sham-control group (G1, n = 10): a sham operation was performed (except for liver I/R). I/R-untreated group (G2, n = 10): rats underwent liver ischemia for 1 h followed by reperfusion for 45 min. I/R-L-Arg group (G3, n = 10): after performing the same surgical procedure as in group 2, animals were treated with L-Arg. Liver tissues were taken for determination of malondialdehyde (MDA) levels, and biochemical and histological evaluations were made.

Results: Plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and gamma-glutamyltranspeptidase (GGT) activities were higher in group 2 than in group 3. MDA values and the hepatic injury score decreased in the L-Arg treated group compared to the I/R-untreated group. In group 2, the hepatocytes were swollen with marked vacuolization. Group 3 rats showed well-preserved liver parenchyma, with hepatocytes extending from the central vein. The morphology of the hepatocytes and the sinusoidal structures was normal, without any signs of congestion. Mild Shh positive immunostaining was detected in group 2 animals. The expression of immunoreactive cells was increased markedly in liver tissue from I/R-L-Arg rats.

Conclusion: Our findings suggest that Shh molecules are critical factors in the pathophysiology of inflammatory liver injury induced by I/R. In addition, NO plays an important role in the immunohistochemical expression of these molecules.

Citing Articles

The Hedgehog Signaling Pathway in Ischemic Tissues.

Giarretta I, Gaetani E, Bigossi M, Tondi P, Asahara T, Pola R Int J Mol Sci. 2019; 20(21).

PMID: 31652910 PMC: 6862352. DOI: 10.3390/ijms20215270.

Protective effect of propofol preconditioning on ischemia-reperfusion injury in human hepatocyte.

Zhang Y, Chen Z, Feng N, Tang J, Zhao X, Liu C J Thorac Dis. 2017; 9(3):702-710.

PMID: 28449478 PMC: 5394059. DOI: 10.21037/jtd.2017.02.80.

The Effect of Etoricoxib on Hepatic Ischemia-Reperfusion Injury in Rats.

Kunak C, Kukula O, Mutlu E, Genc F, Peker G, Kuyrukluyildiz U Oxid Med Cell Longev. 2015; 2015:598162.

PMID: 26236425 PMC: 4506808. DOI: 10.1155/2015/598162.

Microparticles carrying Sonic hedgehog favor neovascularization through the activation of nitric oxide pathway in mice.

Benameur T, Soleti R, Porro C, Andriantsitohaina R, Martinez M PLoS One. 2010; 5(9):e12688.

PMID: 20856928 PMC: 2938335. DOI: 10.1371/journal.pone.0012688.

Sonic hedgehog protects cortical neurons against oxidative stress.

Dai R, Zhu S, Xia Y, Mao L, Mei Y, Yao Y Neurochem Res. 2010; 36(1):67-75.

PMID: 20848190 DOI: 10.1007/s11064-010-0264-6.

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