Over-expression of a Hydroxypyruvate Reductase in Methylobacterium Sp. MB200 Enhances Glyoxylate Accumulation

Overview

Journal J Ind Microbiol Biotechnol

Publisher Oxford University Press

Specialty Biotechnology

Date 2007 Jul 27

PMID 17653579

Citations 2

Authors

Pei-Hong Shen

Bo Wu

Affiliations

Soon will be listed here.

Abstract

Methylobacterium sp. MB200 capable of producing glyoxylate from methanol was obtained by enrichment culture using a medium containing methanol as the sole carbon source. A hpr gene that encodes a hydroxypyruvate reductase (HPR) was cloned from this strain and was ligated into the vector pLAFR3 to obtain the recombinant plasmid pLAFRh, which was transferred into M. sp. MB200 to generate an recombinant strain MB201. Homologous expression of hpr under the control of the lacZ promoter led to the enhanced glyoxylate accumulation in cultures of Methylobacterium sp MB201. The yield of glyoxylate reached 14.38 mg/mL, representing nearly a twofold increase when compared with the wild-type strain.

Citing Articles

Comparative Genomic Analysis of a MB200 Isolated from Biogas Digesters Provided New Insights into the Carbon Metabolism of Methylotrophic Bacteria.

Zhang X, Xia L, Liu J, Wang Z, Yang Y, Wu Y Int J Mol Sci. 2023; 24(8).

PMID: 37108681 PMC: 10138955. DOI: 10.3390/ijms24087521.

Biotechnological and agronomic potential of endophytic pink-pigmented methylotrophic Methylobacterium spp.

Dourado M, Camargo Neves A, Santos D, Araujo W Biomed Res Int. 2015; 2015:909016.

PMID: 25861650 PMC: 4377440. DOI: 10.1155/2015/909016.

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