Immunodetection of Thyroid Hormone Receptor (alpha1/alpha2) in the Rat Uterus and Oviduct

Overview

Journal Acta Histochem Cytochem

Specialty Biochemistry

Date 2007 Jul 27

PMID 17653299

Citations 4

Authors

Jale Oner

Hakan Oner

Affiliations

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Abstract

The aim of this study was to investigate the immunolocalization and the existence of thyroid hormone receptors (THR) (alpha1/alpha2) in rat uterus and oviduct. For this purpose 6 female Wistar albino rats found in estrous period were used. Tissue samples fixed in 10% neutral formalin were examined immunohistochemically. Sections were incubated with primary mouse-monoclonal THR (alpha1/alpha2) antibody. In uterus, THR (alpha1/alpha2) immunoreacted strongly with uterine luminal epithelium, endometrial gland epithelium and endometrial stromal cells and, moderately with myometrial smooth muscle. In oviduct, they were observed moderately in the epithelium of the tube and the smooth muscle cells of the muscular layer. In conclusion, the presence of THR in uterus and oviduct suggests that these organs are an active site of thyroid hormones.

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References

Inuwa I, Williams M . Morphometric study on the uterine horn and thyroid gland in hypothyroid, and thyroxine treated hypothyroid rats. J Anat. 1996; 188 ( Pt 2):383-93. PMC: 1167575. View

Mutvei A, Husman B, Andersson G, Nelson B . Thyroid hormone and not growth hormone is the principle regulator of mammalian mitochondrial biogenesis. Acta Endocrinol (Copenh). 1989; 121(2):223-8. DOI: 10.1530/acta.0.1210223. View

Mutvei A, KUZELA S, Nelson B . Control of mitochondrial transcription by thyroid hormone. Eur J Biochem. 1989; 180(1):235-40. DOI: 10.1111/j.1432-1033.1989.tb14638.x. View

Sap J, Munoz A, Damm K, Goldberg Y, Ghysdael J, Leutz A . The c-erb-A protein is a high-affinity receptor for thyroid hormone. Nature. 1986; 324(6098):635-40. DOI: 10.1038/324635a0. View

McKenna N, Lanz R, OMalley B . Nuclear receptor coregulators: cellular and molecular biology. Endocr Rev. 1999; 20(3):321-44. DOI: 10.1210/edrv.20.3.0366. View

Hastings M, Milcarek C, Martincic K, Peterson M, Munroe S . Expression of the thyroid hormone receptor gene, erbAalpha, in B lymphocytes: alternative mRNA processing is independent of differentiation but correlates with antisense RNA levels. Nucleic Acids Res. 1997; 25(21):4296-300. PMC: 147039. DOI: 10.1093/nar/25.21.4296. View

Tohei A, Imai A, Watanabe G, Taya K . Influence of thiouracil-induced hypothyroidism on adrenal and gonadal functions in adult female rats. J Vet Med Sci. 1998; 60(4):439-46. DOI: 10.1292/jvms.60.439. View

Goglia F, Moreno M, Lanni A . Action of thyroid hormones at the cellular level: the mitochondrial target. FEBS Lett. 1999; 452(3):115-20. DOI: 10.1016/s0014-5793(99)00642-0. View

Wrutniak C, Cassar-Malek I, Marchal S, Rascle A, Heusser S, Keller J . A 43-kDa protein related to c-Erb A alpha 1 is located in the mitochondrial matrix of rat liver. J Biol Chem. 1995; 270(27):16347-54. DOI: 10.1074/jbc.270.27.16347. View

10.

Steinsapir J, Rojas A, Mena M, Tchernitchin A . Effects of thyroid hormone on some uterine responses to estrogen. Endocrinology. 1982; 110(5):1773-9. DOI: 10.1210/endo-110-5-1773. View

11.

Rachez C, Freedman L . Mediator complexes and transcription. Curr Opin Cell Biol. 2001; 13(3):274-80. DOI: 10.1016/s0955-0674(00)00209-x. View

12.

Inuwa I, Williams M . A morphometric study on the endometrium of rat uterus in hypothyroid and thyroxine treated hypothyroid rats. Ups J Med Sci. 2006; 111(2):215-25. DOI: 10.3109/2000-1967-042. View

13.

Baksi S . Effect of propylthiouracil-induced hypothyroidism on serum levels of luteinizing hormone and follicle-stimulating hormone in the rat. J Endocrinol. 1973; 59(3):655-6. DOI: 10.1677/joe.0.0590655. View

14.

Galton V, Martinez E, Hernandez A, St Germain E, Bates J, St Germain D . The type 2 iodothyronine deiodinase is expressed in the rat uterus and induced during pregnancy. Endocrinology. 2001; 142(5):2123-8. DOI: 10.1210/endo.142.5.8169. View

15.

Bassett J, Harvey C, Williams G . Mechanisms of thyroid hormone receptor-specific nuclear and extra nuclear actions. Mol Cell Endocrinol. 2004; 213(1):1-11. DOI: 10.1016/j.mce.2003.10.033. View

16.

Morel G, Ricard-Blum S, Ardail D . Kinetics of internalization and subcellular binding sites for T3 in mouse liver. Biol Cell. 1996; 86(2-3):167-74. DOI: 10.1016/0248-4900(96)84781-2. View

17.

Vienonen A, Miettinen S, Blauer M, Martikainen P, Tomas E, Heinonen P . Expression of nuclear receptors and cofactors in human endometrium and myometrium. J Soc Gynecol Investig. 2004; 11(2):104-12. DOI: 10.1016/j.jsgi.2003.09.003. View

18.

Casas F, Rochard P, Rodier A, Cassar-Malek I, Marchal-Victorion S, Wiesner R . A variant form of the nuclear triiodothyronine receptor c-ErbAalpha1 plays a direct role in regulation of mitochondrial RNA synthesis. Mol Cell Biol. 1999; 19(12):7913-24. PMC: 84876. DOI: 10.1128/MCB.19.12.7913. View

19.

Jain J, Li A, Yang W, Minoo P, Felix J . Mifepristone alters expression of endometrial steroid receptors and their cofactors in new users of medroxyprogesterone acetate. Fertil Steril. 2006; 87(1):8-23. DOI: 10.1016/j.fertnstert.2006.05.076. View

20.

Dunn J, Peppler R, Hess M, Johnson D . Proestrous gonadotropin levels in thyroparathyroidectomized female rats. Experientia. 1976; 32(10):1342-4. DOI: 10.1007/BF01953129. View