High-affinity Interaction Between the S-layer Protein SbsC and the Secondary Cell Wall Polymer of Geobacillus Stearothermophilus ATCC 12980 Determined by Surface Plasmon Resonance Technology

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2007 Jul 24

PMID 17644609

Citations 11

Authors

Judith Ferner-Ortner

Christoph Mader

Nicola Ilk

Uwe B Sleytr

Eva M Egelseer

Affiliations

Soon will be listed here.

Abstract

Surface plasmon resonance studies using C-terminal truncation forms of the S-layer protein SbsC (recombinant SbsC consisting of amino acids 31 to 270 [rSbsC(31-270)] and rSbsC(31-443)) and the secondary cell wall polymer (SCWP) isolated from Geobacillus stearothermophilus ATCC 12980 confirmed the exclusive responsibility of the N-terminal region comprising amino acids 31 to 270 for SCWP binding. Quantitative analyses indicated binding behavior demonstrating low, medium, and high affinities.

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