MglA Regulates Francisella Tularensis Subsp. Novicida (Francisella Novicida) Response to Starvation and Oxidative Stress

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2007 Jul 24

PMID 17644593

Citations 35

Authors

Tina Guina

Dragan Radulovic

Arya J Bahrami

Diana L Bolton

Laurence Rohmer

Kendan A Jones-Isaac

Jinzy Chen

Larry A Gallagher

Byron Gallis

Soyoung Ryu

Greg K Taylor

Mitchell J Brittnacher

Colin Manoil

David R Goodlett

Affiliations

Soon will be listed here.

Abstract

MglA is a transcriptional regulator of genes that contribute to the virulence of Francisella tularensis, a highly infectious pathogen and the causative agent of tularemia. This study used a label-free shotgun proteomics method to determine the F. tularensis subsp. novicida (F. novicida) proteins that are regulated by MglA. The differences in relative protein amounts between wild-type F. novicida and the mglA mutant were derived directly from the average peptide precursor ion intensity values measured with the mass spectrometer by using a suite of mathematical algorithms. Among the proteins whose relative amounts changed in an F. novicida mglA mutant were homologs of oxidative and general stress response proteins. The F. novicida mglA mutant exhibited decreased survival during stationary-phase growth and increased susceptibility to killing by superoxide generated by the redox-cycling agent paraquat. The F. novicida mglA mutant also showed increased survival upon exposure to hydrogen peroxide, likely due to increased amounts of the catalase KatG. Our results suggested that MglA coordinates the stress response of F. tularensis and is likely essential for bacterial survival in harsh environments.

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