Characterization of an Immunoreactive Species-specific 19-kilodalton Outer Membrane Protein from Helicobacter Pylori by Using a Monoclonal Antibody

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1991 Aug 1

PMID 1761682

Citations 12

Authors

E B Drouet

G A Denoyel

M Boude

E Wallano

M Andujar

H P De Montclos

Affiliations

Soon will be listed here.

Abstract

Immunoblotting experiments on hyperimmune rabbit serum and sera from patients with Helicobacter pylori gastritis showed a consistent antibody response to a 19-kDa outer membrane protein antigen. A monoclonal antibody, designated HP 40, which reacted by Western immunoblotting with this protein was produced. It was shared by all H. pylori strains tested (D 273, NCTC 11637, and 24 wild strains) but not by the thermophilic Campylobacter species, Campylobacter fetus, Helicobacter mustellae, or Helicobacter fennelliae. Immunogold staining suggested that the 19-kDa antigen was exposed on the outer surface of the bacteria. Its functional role and effectiveness as a serological diagnostic tool are under study.

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References

Perez-Perez G, Blaser M . Conservation and diversity of Campylobacter pyloridis major antigens. Infect Immun. 1987; 55(5):1256-63. PMC: 260499. DOI: 10.1128/iai.55.5.1256-1263.1987. View

Shulman M, Wilde C, Kohler G . A better cell line for making hybridomas secreting specific antibodies. Nature. 1978; 276(5685):269-70. DOI: 10.1038/276269a0. View

Perez-Perez G, Dworkin B, Chodos J, Blaser M . Campylobacter pylori antibodies in humans. Ann Intern Med. 1988; 109(1):11-7. DOI: 10.7326/0003-4819-109-1-11. View

Vandamme P, Falsen E, Rossau R, Hoste B, Segers P, Tytgat R . Revision of Campylobacter, Helicobacter, and Wolinella taxonomy: emendation of generic descriptions and proposal of Arcobacter gen. nov. Int J Syst Bacteriol. 1991; 41(1):88-103. DOI: 10.1099/00207713-41-1-88. View

Hawtin P, Stacey A, Newell D . Investigation of the structure and localization of the urease of Helicobacter pylori using monoclonal antibodies. J Gen Microbiol. 1990; 136(10):1995-2000. DOI: 10.1099/00221287-136-10-1995. View

Clayton C, Wren B, Mullany P, Topping A, TABAQCHALI S . Molecular cloning and expression of Campylobacter pylori species-specific antigens in Escherichia coli K-12. Infect Immun. 1989; 57(2):623-9. PMC: 313142. DOI: 10.1128/iai.57.2.623-629.1989. View

Dunn B, Perez-Perez G, Blaser M . Two-dimensional gel electrophoresis and immunoblotting of Campylobacter pylori proteins. Infect Immun. 1989; 57(6):1825-33. PMC: 313362. DOI: 10.1128/iai.57.6.1825-1833.1989. View

Czinn S, Carr H, Sheffler L, ARONOFF S . Serum IgG antibody to the outer membrane proteins of Campylobacter pylori in children with gastroduodenal disease. J Infect Dis. 1989; 159(3):586-9. DOI: 10.1093/infdis/159.3.586. View

Glupczynski Y, Burette A, Labbe M, Deprez C, De Reuck M, Deltenre M . Campylobacter pylori-associated gastritis: a double-blind placebo-controlled trial with amoxycillin. Am J Gastroenterol. 1988; 83(4):365-72. View

10.

Apel I, Jacobs E, Kist M, BREDT W . Antibody response of patients against a 120 kDa surface protein of Campylobacter pylori. Zentralbl Bakteriol Mikrobiol Hyg A. 1988; 268(2):271-6. DOI: 10.1016/s0176-6724(88)80012-9. View

11.

von Wulffen H, Grote H, Gatermann S, Loning T, Berger B, Buhl C . Immunoblot analysis of immune response to Campylobacter pylori and its clinical associations. J Clin Pathol. 1988; 41(6):653-9. PMC: 1141546. DOI: 10.1136/jcp.41.6.653. View

12.

Newell D . Identification of the outer membrane proteins of Campylobacter pyloridis and antigenic cross-reactivity between C. pyloridis and C. jejuni. J Gen Microbiol. 1987; 133(1):163-70. DOI: 10.1099/00221287-133-1-163. View

13.

Krakowka S, Morgan D, Kraft W, Leunk R . Establishment of gastric Campylobacter pylori infection in the neonatal gnotobiotic piglet. Infect Immun. 1987; 55(11):2789-96. PMC: 259978. DOI: 10.1128/iai.55.11.2789-2796.1987. View

14.

Goodwin C, Blincow E, Peterson G, Sanderson C, Cheng W, Marshall B . Enzyme-linked immunosorbent assay for Campylobacter pyloridis: correlation with presence of C. pyloridis in the gastric mucosa. J Infect Dis. 1987; 155(3):488-94. DOI: 10.1093/infdis/155.3.488. View

15.

Rathbone B, Wyatt J, Heatley R . Campylobacter pyloridis--a new factor in peptic ulcer disease?. Gut. 1986; 27(6):635-41. PMC: 1433327. DOI: 10.1136/gut.27.6.635. View

16.

Blaser M . Helicobacter pylori and the pathogenesis of gastroduodenal inflammation. J Infect Dis. 1990; 161(4):626-33. DOI: 10.1093/infdis/161.4.626. View

17.

Filip C, Fletcher G, Wulff J, Earhart C . Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate. J Bacteriol. 1973; 115(3):717-22. PMC: 246312. DOI: 10.1128/jb.115.3.717-722.1973. View

18.

Ofarrell P . High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975; 250(10):4007-21. PMC: 2874754. View

19.

Oakley B, Kirsch D, Morris N . A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal Biochem. 1980; 105(2):361-3. DOI: 10.1016/0003-2697(80)90470-4. View

20.

Warren J, Marshall B . Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet. 1983; 1(8336):1273-5. View