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Use of an Escherichia Coli Recombinant Producing Thermostable Polyphosphate Kinase As an ATP Regenerator to Produce Fructose 1,6-diphosphate

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Date 2007 Jul 10
PMID 17616610
Citations 15
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Abstract

Heat-treated Escherichia coli producing Thermus polyphosphate kinase regenerated ATP by using exogenous polyphosphate. This recombinant could be used as a platform to produce valuable compounds in combination with thermostable phosphorylating or energy-requiring enzymes. In this work, we demonstrated the production of fructose 1,6-diphosphate from fructose and polyphosphate.

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