Functional Characterization of Two Melanocortin (MC) Receptors in Lamprey Showing Orthology to the MC1 and MC4 Receptor Subtypes

Overview

Journal BMC Evol Biol

Publisher Biomed Central

Specialty Biology

Date 2007 Jul 3

PMID 17603878

Citations 17

Authors

Tatjana Haitina

Janis Klovins

Akiyoshi Takahashi

Maja Lowgren

Aneta Ringholm

Johan Enberg

Hiroshi Kawauchi

Earl T Larson

Robert Fredriksson

Helgi B Schioth

Affiliations

Soon will be listed here.

Abstract

Background: The melanocortin (MC) receptors have a key role in regulating body weight and pigmentation. They belong to the rhodopsin family of G protein-coupled receptors (GPCRs). The purpose of this study was to identify ancestral MC receptors in agnathan, river lamprey.

Results: We report cloning of two MC receptors from river lamprey. The lamprey receptors, designated MCa and MCb, showed orthology to the MC1 and MC4 receptor subtypes, respectively. The molecular clock analysis suggested that lamprey MC receptor genes were not duplicated recently and diverged from each other more than 400 MYR ago. Expression and pharmacological characterization showed that the lamprey MCa receptor was able to bind and be activated by both lamprey and human MSH peptides. The lamprey MCa receptor had relatively high affinity for ACTH derived peptides similarly to the fish MC receptors. We found that both of the lamprey MC receptors were expressed in skin, while the MCb receptor was also found in liver, heart and skeletal muscle.

Conclusion: This study shows presence of MC receptors in agnathans indicating early signs of specific functions of melanocortin receptor subtypes.

Citing Articles

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