Sortase C-mediated Anchoring of BasI to the Cell Wall Envelope of Bacillus Anthracis

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2007 Jun 26

PMID 17586639

Citations 20

Authors

Luciano A Marraffini

Olaf Schneewind

Affiliations

Soon will be listed here.

Abstract

Vegetative forms of Bacillus anthracis replicate in tissues of an infected host and precipitate lethal anthrax disease. Upon host death, bacilli form dormant spores that contaminate the environment, thereby gaining entry into new hosts where spores germinate and once again replicate as vegetative forms. We show here that sortase C, an enzyme that is required for the formation of infectious spores, anchors BasI polypeptide to the envelope of predivisional sporulating bacilli. BasI anchoring to the cell wall requires the active site cysteine of sortase C and an LPNTA motif sorting signal at the C-terminal end of the BasI precursor. The LPNTA motif of BasI is cleaved between the threonine (T) and the alanine (A) residue; the C-terminal carboxyl group of threonine is subsequently amide linked to the side chain amino group of diaminopimelic acid within the wall peptides of B. anthracis peptidoglycan.

Citing Articles

Structural and functional insights of sortases and their interactions with antivirulence compounds.

Sivaramalingam S, Jothivel D, Govindarajan D, Kadirvelu L, Sivaramakrishnan M, Chithiraiselvan D Curr Res Struct Biol. 2024; 8:100152.

PMID: 38989133 PMC: 11231552. DOI: 10.1016/j.crstbi.2024.100152.

The divergent roles of sortase in the biology of Gram-positive bacteria.

Susmitha A, Bajaj H, Nampoothiri K Cell Surf. 2021; 7:100055.

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Uncovering Roles of Streptococcus gordonii SrtA-Processed Proteins in the Biofilm Lifestyle.

Nairn B, Lee G, Chumber A, Steck P, Mire M, Lima B J Bacteriol. 2020; 203(2).

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Structure of the Bacillus anthracis Sortase A Enzyme Bound to Its Sorting Signal: A FLEXIBLE AMINO-TERMINAL APPENDAGE MODULATES SUBSTRATE ACCESS.

Chan A, Yi S, Terwilliger A, Maresso A, Jung M, Clubb R J Biol Chem. 2015; 290(42):25461-74.

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