SrfI, a New Type-II Restriction Endonuclease That Recognizes the Octanucleotide Sequence, [sequence: See Text]

Overview

Journal Gene

Specialty Molecular Biology

Date 1991 Dec 20

PMID 1756971

Citations 4

Authors

T G Simcox

S J Marsh

E A GROSS

W Lernhardt

S Davis

M E Simcox

Affiliations

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Abstract

A new restriction endonuclease, SrfI has been isolated from an unidentified species of Streptomyces. SrfI recognizes the 8-bp palindrome, 5'-GCCCGGGC and cleaves double-stranded DNA after the third C in the sequence, producing blunt ends. SrfI is a rare-cutting enzyme and should therefore be useful for megabase mapping.

Citing Articles

Targeted high-throughput sequencing of tagged nucleic acid samples.

Meyer M, Stenzel U, Myles S, Prufer K, Hofreiter M Nucleic Acids Res. 2007; 35(15):e97.

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A unique type II restriction endonuclease FspAI, that recognizes the octanucleotide sequence 5'-RTGC/GCAY-3'.

Kesminiene A, Maneliene Z, Vitkute J, Petrusyte M, Janulaitis A Nucleic Acids Res. 2002; 29(24):E120.

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Compilation of small RNA sequences.

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Restriction enzymes and their isoschizomers.

Roberts R, Macelis D Nucleic Acids Res. 1992; 20 Suppl:2167-80.

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