Molecular Cloning and Characterization of Hearm Caspase-1 from Helicoverpa Armigera

Overview

Journal Mol Biol Rep

Specialty Molecular Biology

Date 2007 Jun 2

PMID 17541728

Citations 7

Authors

Dantong Yang

Lianqin Chai

Jinxing Wang

Xiaofan Zhao

Affiliations

Soon will be listed here.

Abstract

Members of the caspase family play a central and evolutionary role in programmed cell death (PCD), which removes unwanted, damaged and dangerous cells during development to maintain homeostasis. In this paper, we describe the cloning and characterization of a caspase from the cotton bollworm, Helicoverpa armigera, named Hearm caspase-1. The 1,350 bp full-length cDNA contains an 885 bp open reading frame (ORF) that encodes a Hearm caspase-1 proenzyme of 294 amino acids. The deduced protein is highly homologous to Spodoptera frugiperda Sf caspase-1 and Drosophila melanogaster ICE and has the highly conserved pentapeptide QACQG, the recognized catalytic site of caspases, suggesting that it is an effector caspase of the cotton bollworm. Northern blot and RT-PCR analyses demonstrate that Hearm caspase-1 is expressed in embryos and the fat body, midgut and haemocytes of feeding and wandering larvae. Expression of Hearm caspase-1 in the haemocytes appears to be correlated with the pulse of ecdysone, and it is up-regulated by ecdysone agonist RH-2485, implying that Hearm caspase-1 activation is regulated by ecdysone.

Citing Articles

Evolutionary novelty in the apoptotic pathway of aphids.

Ribeiro Lopes M, Parisot N, Gaget K, Huygens C, Peignier S, Duport G Proc Natl Acad Sci U S A. 2020; 117(51):32545-32556.

PMID: 33288705 PMC: 7768685. DOI: 10.1073/pnas.2013847117.

Protein kinase C delta phosphorylates ecdysone receptor B1 to promote gene expression and apoptosis under 20-hydroxyecdysone regulation.

Chen C, Pan J, Di Y, Liu W, Hou L, Wang J Proc Natl Acad Sci U S A. 2017; 114(34):E7121-E7130.

PMID: 28790182 PMC: 5576805. DOI: 10.1073/pnas.1704999114.

Rice ragged stunt virus-induced apoptosis affects virus transmission from its insect vector, the brown planthopper to the rice plant.

Huang H, Bao Y, Lao S, Huang X, Ye Y, Wu J Sci Rep. 2015; 5:11413.

PMID: 26073458 PMC: 4466780. DOI: 10.1038/srep11413.

Juvenile hormone prevents 20-hydroxyecdysone-induced metamorphosis by regulating the phosphorylation of a newly identified broad protein.

Cai M, Liu W, Pei X, Li X, He H, Wang J J Biol Chem. 2014; 289(38):26630-26641.

PMID: 25096576 PMC: 4176204. DOI: 10.1074/jbc.M114.581876.

Molecular cloning and characterization of the first caspase in the Striped Stem Borer, Chilo suppressalis.

Lu M, Du Y, Cao S, Liu P, Li J Int J Mol Sci. 2013; 14(5):10229-41.

PMID: 23676354 PMC: 3676837. DOI: 10.3390/ijms140510229.

References

Arbeitman M, Furlong E, Imam F, Johnson E, Null B, Baker B . Gene expression during the life cycle of Drosophila melanogaster. Science. 2002; 297(5590):2270-5. DOI: 10.1126/science.1072152. View

Kumar S, Cakouros D . Transcriptional control of the core cell-death machinery. Trends Biochem Sci. 2004; 29(4):193-9. DOI: 10.1016/j.tibs.2004.02.001. View

Yin V, Thummel C . Mechanisms of steroid-triggered programmed cell death in Drosophila. Semin Cell Dev Biol. 2005; 16(2):237-43. DOI: 10.1016/j.semcdb.2004.12.007. View

Dorstyn L, Colussi P, Quinn L, Richardson H, Kumar S . DRONC, an ecdysone-inducible Drosophila caspase. Proc Natl Acad Sci U S A. 1999; 96(8):4307-12. PMC: 16328. DOI: 10.1073/pnas.96.8.4307. View

Kumar S, Doumanis J . The fly caspases. Cell Death Differ. 2001; 7(11):1039-44. DOI: 10.1038/sj.cdd.4400756. View

Kilpatrick Z, Cakouros D, Kumar S . Ecdysone-mediated up-regulation of the effector caspase DRICE is required for hormone-dependent apoptosis in Drosophila cells. J Biol Chem. 2005; 280(12):11981-6. DOI: 10.1074/jbc.M413971200. View

Thummel C . Steroid-triggered death by autophagy. Bioessays. 2001; 23(8):677-82. DOI: 10.1002/bies.1096. View

Jiang C, Lamblin A, Steller H, Thummel C . A steroid-triggered transcriptional hierarchy controls salivary gland cell death during Drosophila metamorphosis. Mol Cell. 2000; 5(3):445-55. DOI: 10.1016/s1097-2765(00)80439-6. View

Baehrecke E . How death shapes life during development. Nat Rev Mol Cell Biol. 2002; 3(10):779-87. DOI: 10.1038/nrm931. View

10.

Yang X, Hou L, Dong D, Shao H, Wang J, Zhao X . Cathepsin B-like proteinase is involved in the decomposition of the adult fat body of Helicoverpa armigera. Arch Insect Biochem Physiol. 2006; 62(1):1-10. DOI: 10.1002/arch.20115. View

11.

Pei Z, Reske G, Huang Q, Hammock B, Qi Y, Chejanovsky N . Characterization of the apoptosis suppressor protein P49 from the Spodoptera littoralis nucleopolyhedrovirus. J Biol Chem. 2002; 277(50):48677-84. DOI: 10.1074/jbc.M208810200. View

12.

Muller F, Adori C, Sass M . Autophagic and apoptotic features during programmed cell death in the fat body of the tobacco hornworm (Manduca sexta). Eur J Cell Biol. 2004; 83(2):67-78. DOI: 10.1078/0171-9335-00359. View

13.

Fraser A, Evan G . Identification of a Drosophila melanogaster ICE/CED-3-related protease, drICE. EMBO J. 1997; 16(10):2805-13. PMC: 1169889. DOI: 10.1093/emboj/16.10.2805. View

14.

Riddiford L, Hiruma K, Zhou X, Nelson C . Insights into the molecular basis of the hormonal control of molting and metamorphosis from Manduca sexta and Drosophila melanogaster. Insect Biochem Mol Biol. 2003; 33(12):1327-38. DOI: 10.1016/j.ibmb.2003.06.001. View

15.

Kang C, Wang J, Zhao X, Yang X, Shao H, Xiang J . Molecular cloning and expression analysis of Ch-penaeidin, an antimicrobial peptide from Chinese shrimp, Fenneropenaeus chinensis. Fish Shellfish Immunol. 2004; 16(4):513-25. DOI: 10.1016/j.fsi.2003.09.003. View

16.

Fernandes-Alnemri T, Armstrong R, Krebs J, Srinivasula S, Wang L, Bullrich F . In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains. Proc Natl Acad Sci U S A. 1996; 93(15):7464-9. PMC: 38767. DOI: 10.1073/pnas.93.15.7464. View

17.

Truman J, Riddiford L . Endocrine insights into the evolution of metamorphosis in insects. Annu Rev Entomol. 2001; 47:467-500. DOI: 10.1146/annurev.ento.47.091201.145230. View

18.

Xu Y, Kawasaki H . Isolation and expression of cathepsin B cDNA in hemocytes during metamorphosis of Bombyx mori. Comp Biochem Physiol B Biochem Mol Biol. 2001; 130(3):393-9. DOI: 10.1016/s1096-4959(01)00448-1. View

19.

Ahmad M, Srinivasula S, Wang L, Litwack G, Fernandes-Alnemri T, Alnemri E . Spodoptera frugiperda caspase-1, a novel insect death protease that cleaves the nuclear immunophilin FKBP46, is the target of the baculovirus antiapoptotic protein p35. J Biol Chem. 1997; 272(3):1421-4. DOI: 10.1074/jbc.272.3.1421. View

20.

Nicholson D . Caspase structure, proteolytic substrates, and function during apoptotic cell death. Cell Death Differ. 1999; 6(11):1028-42. DOI: 10.1038/sj.cdd.4400598. View