Urinary 8-hydroxydeoxyguanosine is Elevated in Patients with Nephrolithiasis

Overview

Journal Urol Res

Specialty Urology

Date 2007 Jun 2

PMID 17541572

Citations 46

Authors

Chanchai Boonla

Rattiporn Wunsuwan

Kriang Tungsanga

Piyaratana Tosukhowong

Affiliations

Soon will be listed here.

Abstract

8-hydroxydeoxyguanosine (8-OHdG) is an oxidatively modified guanosine, which has been widely used as an oxidative DNA damage marker in various diseases. The present study aimed to determine urinary 8-OHdG in nephrolithiasis patients and evaluate its clinical significance. Thirty-six nephrolithiasis patients and 30 healthy subjects were recruited. Urine volume, creatinine, malondialdehyde, beta-N-acetylglucosaminidase (NAG) activity and proteins were measured in 24 h urine samples. Urinary 8-OHdG was determined by competitive enzyme-linked immunosorbent assay. Mineral composition of stones was analyzed using Fourier-transformed infrared spectroscopy. Nephrolithiasis patients excreted urinary 8-OHdG significantly higher than healthy controls. Urinary 8-OHdG levels compared among patients with calcium oxalate, struvite and uric acid stones were insignificantly different. The urinary NAG activity correlated positively with urinary 8-OHdG. Multiple linear regression showed that urinary NAG activity was an independent predictor of urinary 8-OHdG level. Receiver operating characteristic analysis revealed that the urinary 8-OHdG test was adequate for diagnosing nephrolithiasis. At 10 mug/g creatinine cutoff, the 8-OHdG test imparted high specificity (96.67%) and a positive predictive value (91.67%). In conclusion, this is the first report of elevated urinary 8-OHdG excretion in nephrolithiasis patients indicating increased oxidative DNA damage. Increased renal tubular damage was independently associated with elevated urinary 8-OHdG. Elevated urinary 8-OHdG levels adjunct with metabolic profile may be useful for identifying people at risk of stone development.

Citing Articles

Association between the oxidative balance score and kidney stones in adults.

Chen Q, Bao W, Kong X, Zhu J, Hou S, Zhang Y World J Urol. 2024; 42(1):425.

PMID: 39037613 DOI: 10.1007/s00345-024-05144-5.

A Nuclear Magnetic Resonance (NMR)- and Mass Spectrometry (MS)-Based Saturation Kinetics Model of a Decoction as a Treatment for Kidney Stones.

Chik C, Larroque A, Zhuang Y, Feinstein S, Smith D, Andonian S Int J Mol Sci. 2024; 25(10).

PMID: 38791318 PMC: 11121557. DOI: 10.3390/ijms25105280.

A prospective case-control study on the evaluation of oxidative stress in renal stone formers.

Manoharan V, Sharma G, Devana S, Sharma S, Avti P, Chandramouli S Urolithiasis. 2024; 52(1):18.

PMID: 38165517 DOI: 10.1007/s00240-023-01514-7.

Polymorphic variations and mRNA expression of the genes encoding interleukins as well as enzymes of oxidative and nitrative stresses as a potential risk of nephrolithiasis development.

Wigner-Jeziorska P, Grebowski R, Saluk J, Bijak M, Szemraj J PLoS One. 2023; 18(10):e0293280.

PMID: 37878647 PMC: 10599546. DOI: 10.1371/journal.pone.0293280.

The Protective Role of Dietary Polyphenols in Urolithiasis: Insights into Antioxidant Effects and Mechanisms of Action.

Hong S, Qin B Nutrients. 2023; 15(17).

PMID: 37686790 PMC: 10490426. DOI: 10.3390/nu15173753.

References

Huang H, Ma M, Chen C, Chen J . Lipid peroxidation and its correlations with urinary levels of oxalate, citric acid, and osteopontin in patients with renal calcium oxalate stones. Urology. 2003; 62(6):1123-8. DOI: 10.1016/s0090-4295(03)00764-7. View

Marshall P, Warso M, Lands W . Selective microdetermination of lipid hydroperoxides. Anal Biochem. 1985; 145(1):192-9. DOI: 10.1016/0003-2697(85)90347-1. View

Thamilselvan S, Khan S . Oxalate and calcium oxalate crystals are injurious to renal epithelial cells: results of in vivo and in vitro studies. J Nephrol. 1998; 11 Suppl 1:66-9. View

Pilger A, Germadnik D, Riedel K, Scherer G, Rudiger H . Longitudinal study of urinary 8-hydroxy-2'-deoxyguanosine excretion in healthy adults. Free Radic Res. 2001; 35(3):273-80. DOI: 10.1080/10715760100300811. View

Horak E, Hopfer S, SUNDERMAN Jr F . Spectrophotometric assay for urinary N-acetyl-beta-D-glucosaminidase activity. Clin Chem. 1981; 27(7):1180-5. View

Cooke M, Evans M, Dizdaroglu M, Lunec J . Oxidative DNA damage: mechanisms, mutation, and disease. FASEB J. 2003; 17(10):1195-214. DOI: 10.1096/fj.02-0752rev. View

Thamilselvan S, Khan S, Menon M . Oxalate and calcium oxalate mediated free radical toxicity in renal epithelial cells: effect of antioxidants. Urol Res. 2003; 31(1):3-9. DOI: 10.1007/s00240-002-0286-x. View

Toraason M, Butler M, Ruder A, Forrester C, Taylor L, Ashley D . Effect of perchloroethylene, smoking, and race on oxidative DNA damage in female dry cleaners. Mutat Res. 2003; 539(1-2):9-18. DOI: 10.1016/s1383-5718(03)00130-x. View

Besaratinia A, Besarati Nia A, Schooten F, Schilderman P, de Kok T, Haenen G . A multi-biomarker approach to study the effects of smoking on oxidative DNA damage and repair and antioxidative defense mechanisms. Carcinogenesis. 2001; 22(3):395-401. DOI: 10.1093/carcin/22.3.395. View

10.

Faure H, Mousseau M, Cadet J, Guimier C, Tripier M, Hida H . Urine 8-oxo-7,8-dihydro-2-deoxyguanosine vs. 5-(hydroxymethyl) uracil as DNA oxidation marker in adriamycin-treated patients. Free Radic Res. 1998; 28(4):377-82. DOI: 10.3109/10715769809070806. View

11.

Wu L, Chiou C, Chang P, Wu J . Urinary 8-OHdG: a marker of oxidative stress to DNA and a risk factor for cancer, atherosclerosis and diabetics. Clin Chim Acta. 2003; 339(1-2):1-9. DOI: 10.1016/j.cccn.2003.09.010. View

12.

Erhola M, Toyokuni S, Okada K, Tanaka T, Hiai H, Ochi H . Biomarker evidence of DNA oxidation in lung cancer patients: association of urinary 8-hydroxy-2'-deoxyguanosine excretion with radiotherapy, chemotherapy, and response to treatment. FEBS Lett. 1997; 409(2):287-91. DOI: 10.1016/s0014-5793(97)00523-1. View

13.

Khan S . Hyperoxaluria-induced oxidative stress and antioxidants for renal protection. Urol Res. 2005; 33(5):349-57. DOI: 10.1007/s00240-005-0492-4. View

14.

Chiou C, Chang P, Chan E, Wu T, Tsao K, Wu J . Urinary 8-hydroxydeoxyguanosine and its analogs as DNA marker of oxidative stress: development of an ELISA and measurement in both bladder and prostate cancers. Clin Chim Acta. 2003; 334(1-2):87-94. DOI: 10.1016/s0009-8981(03)00191-8. View

15.

Huang H, Ma M, Chen J, Chen C . Changes in the oxidant-antioxidant balance in the kidney of rats with nephrolithiasis induced by ethylene glycol. J Urol. 2002; 167(6):2584-93. View

16.

Huang M, Chaturvedi L, Koul S, Koul H . Oxalate stimulates IL-6 production in HK-2 cells, a line of human renal proximal tubular epithelial cells. Kidney Int. 2005; 68(2):497-503. DOI: 10.1111/j.1523-1755.2005.00427.x. View

17.

Evan A, Lingeman J, Coe F, Worcester E . Randall's plaque: pathogenesis and role in calcium oxalate nephrolithiasis. Kidney Int. 2006; 69(8):1313-8. DOI: 10.1038/sj.ki.5000238. View

18.

Park E, Shigenaga M, Degan P, Korn T, Kitzler J, Wehr C . Assay of excised oxidative DNA lesions: isolation of 8-oxoguanine and its nucleoside derivatives from biological fluids with a monoclonal antibody column. Proc Natl Acad Sci U S A. 1992; 89(8):3375-9. PMC: 48870. DOI: 10.1073/pnas.89.8.3375. View

19.

Kimura S, Yamauchi H, Hibino Y, Iwamoto M, Sera K, Ogino K . Evaluation of urinary 8-hydroxydeoxyguanine in healthy Japanese people. Basic Clin Pharmacol Toxicol. 2006; 98(5):496-502. DOI: 10.1111/j.1742-7843.2006.pto_217.x. View

20.

Umekawa T, Chegini N, Khan S . Increased expression of monocyte chemoattractant protein-1 (MCP-1) by renal epithelial cells in culture on exposure to calcium oxalate, phosphate and uric acid crystals. Nephrol Dial Transplant. 2003; 18(4):664-9. DOI: 10.1093/ndt/gfg140. View