Proteomic Identification of Differently Expressed Proteins Responsible for Osteoblast Differentiation from Human Mesenchymal Stem Cells

Overview

Journal Mol Cell Biochem

Publisher Springer

Specialty Biochemistry

Date 2007 May 29

PMID 17530189

Citations 29

Authors

Ai-Xia Zhang

Wei-Hua Yu

Bao-Feng Ma

Xin-Bing Yu

Frank Fuxiang Mao

Wei Liu

Jia-Qing Zhang

Xiu-Ming Zhang

Shu-Nong Li

Ming-Tao Li

Bruce T Lahn

Andy Peng Xiang

Affiliations

Soon will be listed here.

Abstract

Human mesenchymal stem cells (hMSC) are a population of multipotent cells that can differentiate into osteoblasts, chondrocytes, adipocytes, and other cells. The exact mechanism governing the differentiation of hMSC into osteoblasts remains largely unknown. Here, we analyzed protein expression profiles of undifferentiated as well as osteogenic induced hMSC using 2-D gel electrophoresis (2-DE), mass spectrometry (MS), and peptide mass fingerprinting (PMF) to investigate the early gene expression in osteoblast differentiation. We have generated proteome maps of undifferentiated hMSC and osteogenic induced hMSC on day 3 and day 7. 2-DE revealed 102 spots with at least 2.0-fold changes in expression and 52 differently expressed proteins were successfully identified by MALDI-TOF-MS. These proteins were classified into 7 functional categories: metabolism, signal transduction, transcription, calcium-binding protein, protein degradation, protein folding and others. The expression of some identified proteins was confirmed by further RT-PCR analyses. This study clarifies the global proteome during osteoblast differentiation. Our results will play an important role in better elucidating the underlying molecular mechanism in hMSC differentiation into osteoblasts.

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