Activating and Inhibitory Receptors on Synovial Fluid Natural Killer Cells of Arthritis Patients: Role of CD94/NKG2A in Control of Cytokine Secretion

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 2007 May 25

PMID 17521371

Citations 40

Authors

Cristina Teixeira de Matos

Louise Berg

Jakob Michaelsson

Li Fellander-Tsai

Klas Karre

Kalle Soderstrom

Affiliations

Soon will be listed here.

Abstract

Natural killer (NK) cells are activated early during inflammatory events and contribute to the shaping of the ensuing adaptive immune response. To further understand the role for NK cells in inflammation, we investigated the phenotype and function of synovial fluid (SF) NK cells from patients with chronic joint inflammation, as well as from patients with transient inflammation of the knee following trauma. We confirm that synovial NK cells are similar to the well-characterized CD56(bright) peripheral blood (PB) NK-cell subset present in healthy individuals. However, compared to this PB subset the synovial NK cells express a higher degree of activation markers including CD69 and NKp44, the latter being up-regulated also on CD56(bright) NK cells in the PB of patients. Activated synovial NK cells produced interferon-gamma and tumour necrosis factor, and the production was further up-regulated by antibody masking of CD94/NKG2A, and down-regulated by target cells expressing human leucocyte antigen-E in complex with peptides known to engage CD94/NKG2A. We conclude that synovial NK cells have an activated phenotype and that CD94/NKG2A is a key regulator of synovial NK-cell cytokine synthesis.

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