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Increased Detection of Respiratory Syncytial Virus, Influenza Viruses, Parainfluenza Viruses, and Adenoviruses with Real-time PCR in Samples from Patients with Respiratory Symptoms

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 2007 May 18
PMID 17507513
Citations 73
Authors
Alma C van de Pol
Anton M van Loon
Tom F W Wolfs
Nicolaas J G Jansen
Monique Nijhuis
Els Klein Breteler
Rob Schuurman
John W A Rossen
Affiliations
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Abstract

Respiratory samples (n = 267) from hospitalized patients with respiratory symptoms were tested by real-time PCR, viral culture, and direct immunofluorescence for respiratory syncytial virus, influenza virus, parainfluenza viruses, and adenoviruses. Compared with conventional diagnostic tests, real-time PCR increased the diagnostic yields for these viruses from 24% to 43% and from 3.5% to 36% for children and adults, respectively.

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