Isolation and Identification of an Endophytic Strain EJS-3 Producing Novel Fibrinolytic Enzymes

Overview

Journal Curr Microbiol

Specialty Microbiology

Date 2007 May 10

PMID 17487531

Citations 7

Authors

Fengxia Lu

Lijun Sun

Zhaoxin Lu

Xiaomei Bie

Yaowei Fang

Shu Liu

Affiliations

Soon will be listed here.

Abstract

An endophytic strain EJS-3, which produces a novel fibrinolytic enzyme, was screened from root tissue of Stemona japonica (Blume) Miq, a chinese traditional medicine. This strain was identified as Paenibacillus polymyxa (DQ120522) by morphological, physiological, and biochemical tests and 16S rRNA gene sequence analysis. Two serine-type fibrinolytic enzymes with a relative molecular weight about 118 and 49 kDa, respectively, which are larger than known fibrinolytic enzymes, were found by the SDS-fibrin zymogram or by fibrin-inhibitor zymography gels. No work on P. polymyxa-producing fibrinolytic enzymes has been reported.

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References

Chang C, Fan M, Kuo F, Sung H . Potent fibrinolytic enzyme from a mutant of Bacillus subtilis IMR-NK1. J Agric Food Chem. 2000; 48(8):3210-6. DOI: 10.1021/jf000020k. View

Medved L, Solovjov D, Ingham K . Domain structure, stability and interactions in streptokinase. Eur J Biochem. 1996; 239(2):333-9. DOI: 10.1111/j.1432-1033.1996.0333u.x. View

Suto M, Takebayashi M, Saito K, Tanaka M, Yokota A, Tomita F . Endophytes as producers of xylanase. J Biosci Bioeng. 2005; 93(1):88-90. View

Fujita M, Nomura K, Hong K, Ito Y, Asada A, Nishimuro S . Purification and characterization of a strong fibrinolytic enzyme (nattokinase) in the vegetable cheese natto, a popular soybean fermented food in Japan. Biochem Biophys Res Commun. 1993; 197(3):1340-7. DOI: 10.1006/bbrc.1993.2624. View

Kim S, Choi N . Electrophoretic analysis of protease inhibitors in fibrin zymography. Anal Biochem. 1999; 270(1):179-81. DOI: 10.1006/abio.1999.4080. View

Strobel G . Endophytes as sources of bioactive products. Microbes Infect. 2003; 5(6):535-44. DOI: 10.1016/s1286-4579(03)00073-x. View

Matsubara K, Hori K, Matsuura Y, Miyazawa K . A fibrinolytic enzyme from a marine green alga, Codium latum. Phytochemistry. 2000; 52(6):993-9. DOI: 10.1016/s0031-9422(99)00356-8. View

Lukianchuk V, Reva O, Polishchuk L . [Endophytic bacilli--producers of type II restriction endonucleases]. Mikrobiologiia. 2002; 71(4):491-3. View

Goldhaber S, Bounameaux H . Thrombolytic therapy in pulmonary embolism. Semin Vasc Med. 2004; 1(2):213-20. DOI: 10.1055/s-2001-18490. View

10.

Ko J, Yan J, Zhu L, Qi Y . Identification of two novel fibrinolytic enzymes from Bacillus subtilis QK02. Comp Biochem Physiol C Toxicol Pharmacol. 2004; 137(1):65-74. DOI: 10.1016/j.cca.2003.11.008. View

11.

Kim S, Choi N . Purification and characterization of subtilisin DJ-4 secreted by Bacillus sp. strain DJ-4 screened from Doen-Jang. Biosci Biotechnol Biochem. 2000; 64(8):1722-5. DOI: 10.1271/bbb.64.1722. View

12.

Choi N, Kim S . Two fibrin zymography methods for analysis of plasminogen activators on gels. Anal Biochem. 2000; 281(2):236-8. DOI: 10.1006/abio.2000.4572. View

13.

Wun T, Schleuning W, Reich E . Isolation and characterization of urokinase from human plasma. J Biol Chem. 1982; 257(6):3276-83. View

14.

Kim S, Choi N, Lee W . Fibrin zymography: a direct analysis of fibrinolytic enzymes on gels. Anal Biochem. 1998; 263(1):115-6. DOI: 10.1006/abio.1998.2816. View

15.

Nakajima N, Taya N, Sumi H . Potent fibrinolytic enzyme from the lysate of Katsuwonus pelamis digestive tract (Shiokara): purification and characterization. Biosci Biotechnol Biochem. 1993; 57(9):1604-5. DOI: 10.1271/bbb.57.1604. View

16.

ASTRUP T, Mullertz S . The fibrin plate method for estimating fibrinolytic activity. Arch Biochem Biophys. 1952; 40(2):346-51. DOI: 10.1016/0003-9861(52)90121-5. View

17.

Peng Y, Huang Q, Zhang R, Zhang Y . Purification and characterization of a fibrinolytic enzyme produced by Bacillus amyloliquefaciens DC-4 screened from douchi, a traditional Chinese soybean food. Comp Biochem Physiol B Biochem Mol Biol. 2003; 134(1):45-52. DOI: 10.1016/s1096-4959(02)00183-5. View

18.

Kim W, Choi K, Kim Y, Park H, Choi J, Lee Y . Purification and characterization of a fibrinolytic enzyme produced from Bacillus sp. strain CK 11-4 screened from Chungkook-Jang. Appl Environ Microbiol. 1996; 62(7):2482-8. PMC: 168030. DOI: 10.1128/aem.62.7.2482-2488.1996. View

19.

Sasaki K, Moriyama S, Tanaka Y, Sumi H, Toki N, ROBBINS K . The transport of 125I-labeled human high molecular weight urokinase across the intestinal tract in a dog model with stimulation of synthesis and/or release of plasminogen activators. Blood. 1985; 66(1):69-75. View

20.

Kim S, Lee D, Cheigh C, Choe E, Lee S, Hong Y . Purification and characterization of a fibrinolytic subtilisin-like protease of Bacillus subtilis TP-6 from an Indonesian fermented soybean, Tempeh. J Ind Microbiol Biotechnol. 2006; 33(6):436-44. DOI: 10.1007/s10295-006-0085-4. View