Culture of Human Mast Cells from Peripheral Blood Progenitors

Overview

Journal Nat Protoc

Specialties Biology
Pathology
Science

Date 2007 May 10

PMID 17487210

Citations 29

Authors

Hirohisa Saito

Atsushi Kato

Kenji Matsumoto

Yoshimichi Okayama

Affiliations

Soon will be listed here.

Abstract

This protocol details a method for obtaining a considerable number of human mast cells from peripheral blood cells from normal donors without using stem cell mobilization treatment. By using the magnetic cell sorting system, 10(4)-10(5) cells are retrieved in the CD34+ fraction when 100 ml of blood is drawn from a healthy donor. When these cells are cultured using methylcellulose medium supplemented with stem cell factor and interleukin 6, 10(3)-10(4) mast cell colonies are formed in 6 weeks. The total mast cell number at 6 weeks of culture will be 10(6)-10(7).

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