Weak Self-association in a Carbohydrate System

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 2007 May 8

PMID 17483161

Citations 14

Authors

Trushar R Patel

Stephen E Harding

Anna Ebringerova

Marcin Deszczynski

Zdenka Hromadkova

Adiaratou Togola

Berit Smestad Paulsen

Gordon A Morris

Arthur J Rowe

Affiliations

Soon will be listed here.

Abstract

The physiological importance of weak interactions between biological macromolecules (molar dissociation constants >10 microM) is now well recognized, particularly with regard to cell adhesion and immunological phenomena, and many weak interactions have been measured for proteins. The concomitant importance of carbohydrate-carbohydrate interactions has also been identified, although no weak interaction between pure carbohydrate systems has ever been measured. We now demonstrate for the first time to our knowledge using a powerful probe for weak interactions--sedimentation velocity in the analytical ultracentrifuge--that at least some carbohydrates (from the class of polysaccharides known as heteroxylans and demonstrated here to be biologically active) can show well-defined weak self-interactions of the "monomer-dimer" type frequently found in protein systems. The weak interaction between the heteroxylans is shown from a temperature dependence study to be likely to be hydrophobic in nature.

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References

Jumblatt J, Schlup V, Burger M . Cell-cell recognition: specific binding of Microciona sponge aggregation factor to homotypic cells and the role of calcium ions. Biochemistry. 1980; 19(5):1038-42. DOI: 10.1021/bi00546a032. View

Springer T . Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm. Cell. 1994; 76(2):301-14. DOI: 10.1016/0092-8674(94)90337-9. View

Wyer J, Willcox B, Gao G, Gerth U, Davis S, Bell J . T cell receptor and coreceptor CD8 alphaalpha bind peptide-MHC independently and with distinct kinetics. Immunity. 1999; 10(2):219-25. DOI: 10.1016/s1074-7613(00)80022-9. View

Ebringerova A, Hromadkova Z . Effect of ultrasound on the extractibility of corn bran hemicelluloses. Ultrason Sonochem. 2002; 9(4):225-9. DOI: 10.1016/s1350-4177(01)00124-9. View

Eggens I, Fenderson B, Toyokuni T, Dean B, Stroud M, Hakomori S . Specific interaction between Lex and Lex determinants. A possible basis for cell recognition in preimplantation embryos and in embryonal carcinoma cells. J Biol Chem. 1989; 264(16):9476-84. View

Fenderson B, Zehavi U, Hakomori S . A multivalent lacto-N-fucopentaose III-lysyllysine conjugate decompacts preimplantation mouse embryos, while the free oligosaccharide is ineffective. J Exp Med. 1984; 160(5):1591-6. PMC: 2187492. DOI: 10.1084/jem.160.5.1591. View

Menikh A, Nyholm P, Boggs J . Characterization of the interaction of Ca2+ with hydroxy and non-hydroxy fatty acid species of cerebroside sulfate by Fourier transform infrared spectroscopy and molecular modeling. Biochemistry. 1997; 36(12):3438-47. DOI: 10.1021/bi961869q. View

Kanari M, Tomoda M, Gonda R, Shimizu N, Kimura M, Kawaguchi M . A reticuloendothelial system-activating arabinoxylan from the bark of Cinnamomum cassia. Chem Pharm Bull (Tokyo). 1989; 37(12):3191-4. DOI: 10.1248/cpb.37.3191. View

Leishman A, Naidenko O, Attinger A, Koning F, Lena C, Xiong Y . T cell responses modulated through interaction between CD8alphaalpha and the nonclassical MHC class I molecule, TL. Science. 2001; 294(5548):1936-9. DOI: 10.1126/science.1063564. View

10.

Brown M, Boles K, van der Merwe P, Kumar V, Mathew P, Barclay A . 2B4, the natural killer and T cell immunoglobulin superfamily surface protein, is a ligand for CD48. J Exp Med. 1998; 188(11):2083-90. PMC: 2212392. DOI: 10.1084/jem.188.11.2083. View

11.

Vales-Gomez M, Reyburn H, Mandelboim M, Strominger J . Kinetics of interaction of HLA-C ligands with natural killer cell inhibitory receptors. Immunity. 1998; 9(3):337-44. DOI: 10.1016/s1074-7613(00)80616-0. View

12.

Ebringerova A, Kardosova A, Hromadkova Z, Hribalova V . Mitogenic and comitogenic activities of polysaccharides from some European herbaceous plants. Fitoterapia. 2003; 74(1-2):52-61. DOI: 10.1016/s0367-326x(02)00295-2. View

13.

Schuck P, Rossmanith P . Determination of the sedimentation coefficient distribution by least-squares boundary modeling. Biopolymers. 2000; 54(5):328-41. DOI: 10.1002/1097-0282(20001015)54:5<328::AID-BIP40>3.0.CO;2-P. View

14.

Cavallaro U, Christofori G . Cell adhesion and signalling by cadherins and Ig-CAMs in cancer. Nat Rev Cancer. 2004; 4(2):118-32. DOI: 10.1038/nrc1276. View

15.

Xiong Y, Kern P, Chang H, Reinherz E . T Cell Receptor Binding to a pMHCII Ligand Is Kinetically Distinct from and Independent of CD4. J Biol Chem. 2000; 276(8):5659-67. DOI: 10.1074/jbc.M009580200. View

16.

Song Y, Withers D, Hakomori S . Globoside-dependent adhesion of human embryonal carcinoma cells, based on carbohydrate-carbohydrate interaction, initiates signal transduction and induces enhanced activity of transcription factors AP1 and CREB. J Biol Chem. 1998; 273(5):2517-25. DOI: 10.1074/jbc.273.5.2517. View

17.

Bucior I, Burger M . Carbohydrate-carbohydrate interaction as a major force initiating cell-cell recognition. Glycoconj J. 2004; 21(3-4):111-23. DOI: 10.1023/B:GLYC.0000044843.72595.7d. View

18.

Harding S . Challenges for the modern analytical ultracentrifuge analysis of polysaccharides. Carbohydr Res. 2005; 340(5):811-26. DOI: 10.1016/j.carres.2005.01.027. View

19.

Springer T . Adhesion receptors of the immune system. Nature. 1990; 346(6283):425-34. DOI: 10.1038/346425a0. View

20.

Gilbert L, Gilbert G . Sedimentation velocity measurement of protein association. Methods Enzymol. 1973; 27:273-96. DOI: 10.1016/s0076-6879(73)27014-3. View