Neutralizing Antibodies After Infection with Dengue 1 Virus

Overview

Journal Emerg Infect Dis

Specialty Infectious Diseases

Date 2007 May 8

PMID 17479892

Citations 60

Authors

Maria G Guzman

Mayling Alvarez

Rosmari Rodriguez-Roche

Lidice Bernardo

Tibaire Montes

Susana Vazquez

Luis Morier

Angel Alvarez

Ernest A Gould

Gustavo Kouri

Scott B Halstead

Affiliations

Soon will be listed here.

Abstract

Severity of disease is markedly increased when infection with dengue virus type 2 (DENV-2) follows infection with DENV-1 at an interval of 20 years. Studies have shown that heterologous neutralizing antibody titers are inversely correlated with severity of a second infection. If this mechanism controlled disease severity in Cuba, heterotypic antibody titers should have declined over time. To determine whether phenotypic changes in dengue antibodies occur over time, we analyzed serum samples collected 4-8 and 20-22 years after DENV-1 infection. We found a significant increase in mean titer of homologous DENV-1 neutralizing antibodies and a significant decrease in heterologous antibodies to 1 of 2 genotypes of DENV-2 virus (the American genotype). Asian DENV-2 viruses were not neutralized during either interval; however, the American genotype underwent phenotypic changes in heterotypic viral neutralizing antibodies in the predicted direction. This finding may be related to the time-dependent changes in severity of disease found with secondary dengue infection.

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References

Guzman M, Kouri G, Bravo J, Soler M, Vazquez S, Santos M . Dengue haemorrhagic fever in Cuba. II. Clinical investigations. Trans R Soc Trop Med Hyg. 1984; 78(2):239-41. DOI: 10.1016/0035-9203(84)90286-4. View

Kochel T, Watts D, Gozalo A, Ewing D, Porter K, Russell K . Cross-serotype neutralization of dengue virus in Aotus nancymae monkeys. J Infect Dis. 2005; 191(6):1000-4. DOI: 10.1086/427511. View

Rodriguez-Roche R, Alvarez M, Gritsun T, Rosario D, Halstead S, Kouri G . Dengue virus type 2 in Cuba, 1997: conservation of E gene sequence in isolates obtained at different times during the epidemic. Arch Virol. 2004; 150(3):415-25. DOI: 10.1007/s00705-004-0445-1. View

Guzman M, Kouri G, Bravo J, Soler M, Vazquez S, Morier L . Dengue hemorrhagic fever in Cuba, 1981: a retrospective seroepidemiologic study. Am J Trop Med Hyg. 1990; 42(2):179-84. DOI: 10.4269/ajtmh.1990.42.179. View

Guzman M, Kouri G, Valdes L, Bravo J, Alvarez M, Vazques S . Epidemiologic studies on Dengue in Santiago de Cuba, 1997. Am J Epidemiol. 2000; 152(9):793-9; discussion 804. DOI: 10.1093/aje/152.9.793. View

Endy T, Nisalak A, Chunsuttitwat S, Vaughn D, Green S, Ennis F . Relationship of preexisting dengue virus (DV) neutralizing antibody levels to viremia and severity of disease in a prospective cohort study of DV infection in Thailand. J Infect Dis. 2004; 189(6):990-1000. DOI: 10.1086/382280. View

Fernandez R, Vazquez S . Serological diagnosis of dengue by an ELISA inhibition method (EIM). Mem Inst Oswaldo Cruz. 1990; 85(3):347-51. DOI: 10.1590/s0074-02761990000300012. View

Halstead S, GROSZ C . Subclinical Japanese encephalitis. I. Infection of Americans with limited residence in Korea. Am J Hyg. 1962; 75:190-201. View

Guzman M, Kouri G, Valdes L, Bravo J, Vazquez S, Halstead S . Enhanced severity of secondary dengue-2 infections: death rates in 1981 and 1997 Cuban outbreaks. Rev Panam Salud Publica. 2002; 11(4):223-7. DOI: 10.1590/s1020-49892002000400003. View

10.

Kliks S, Nisalak A, BRANDT W, Wahl L, Burke D . Antibody-dependent enhancement of dengue virus growth in human monocytes as a risk factor for dengue hemorrhagic fever. Am J Trop Med Hyg. 1989; 40(4):444-51. DOI: 10.4269/ajtmh.1989.40.444. View

11.

Kochel T, Watts D, Halstead S, Hayes C, Espinoza A, Felices V . Effect of dengue-1 antibodies on American dengue-2 viral infection and dengue haemorrhagic fever. Lancet. 2002; 360(9329):310-2. DOI: 10.1016/S0140-6736(02)09522-3. View

12.

Watts D, PORTER K, Putvatana P, Vasquez B, Calampa C, Hayes C . Failure of secondary infection with American genotype dengue 2 to cause dengue haemorrhagic fever. Lancet. 1999; 354(9188):1431-4. DOI: 10.1016/S0140-6736(99)04015-5. View

13.

Valdes L, Guzman M, Kouri G, Delgado J, Carbonell I, Cabrera M . [Epidemiology of dengue and hemorrhagic dengue in Santiago, Cuba 1997]. Rev Panam Salud Publica. 1999; 6(1):16-25. DOI: 10.1590/s1020-49891999000600003. View

14.

Morens D, Halstead S, Repik P, Putvatana R, Raybourne N . Simplified plaque reduction neutralization assay for dengue viruses by semimicro methods in BHK-21 cells: comparison of the BHK suspension test with standard plaque reduction neutralization. J Clin Microbiol. 1985; 22(2):250-4. PMC: 268369. DOI: 10.1128/jcm.22.2.250-254.1985. View

15.

Kouri G, Guzman M, Bravo J . Hemorrhagic dengue in Cuba: history of an epidemic. Bull Pan Am Health Organ. 1986; 20(1):24-30. View

16.

Guzman M, Kouri G, Bravo J, Soler M, Martinez E . Sequential infection as risk factor for dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS) during the 1981 dengue hemorrhagic Cuban epidemic. Mem Inst Oswaldo Cruz. 1991; 86(3):367. DOI: 10.1590/s0074-02761991000300011. View

17.

Guzman M, Deubel V, Pelegrino J, Rosario D, Marrero M, Sariol C . Partial nucleotide and amino acid sequences of the envelope and the envelope/nonstructural protein-1 gene junction of four dengue-2 virus strains isolated during the 1981 Cuban epidemic. Am J Trop Med Hyg. 1995; 52(3):241-6. DOI: 10.4269/ajtmh.1995.52.241. View

18.

Cantelar de Francisco N, Fernandez A, Albert Molina L, Perez Balbis E . [Survey of dengue in Cuba. 1978-1979]. Rev Cubana Med Trop. 1981; 33(1):72-8. View

19.

Alvarez M, Guzman M, Rosario D, Vazquez S, Pelegrino J, Sariol C . [Direct sequencing of an amplified product from a serum sample]. Rev Cubana Med Trop. 1996; 48(1):53-5. View

20.

RUSSELL P, Nisalak A, Sukhavachana P, VIVONA S . A plaque reduction test for dengue virus neutralizing antibodies. J Immunol. 1967; 99(2):285-90. View