Association of Cytochrome B Translational Activator Proteins with the Mitochondrial Membrane: Implications for Cytochrome B Expression in Yeast

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1991 Nov 1

PMID 1745228

Citations 23

Authors

U Michaelis

A Korte

G Rodel

Affiliations

Soon will be listed here.

Abstract

The products of the nuclear genes CBS1 and CBS2 are both required for translational activation of mitochondrial apocytochrome b in yeast. We report the intramitochondrial localization of both proteins by use of specific antisera. Based on its solubilization properties the CBS1 protein is presumed to be a component of the mitochondrial membrane; the detergent concentrations needed to release CBS1 from mitochondria are almost the same as for cytochrome c1. In contrast, CBS2 behaves like a soluble protein, with some characteristics of a membrane-associated protein. A model is presented for translational activation of cytochrome b, which might also be applicable to translational regulation of other mitochondrial genes.

Citing Articles

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Ribosome-binding proteins Mdm38 and Mba1 display overlapping functions for regulation of mitochondrial translation.

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Saccharomyces cerevisiae translational activator Cbs2p is associated with mitochondrial ribosomes.

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The mitochondrial message-specific mRNA protectors Cbp1 and Pet309 are associated in a high-molecular weight complex.

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