A Yeast Two-hybrid Smart-pool-array System for Protein-interaction Mapping

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2007 Apr 24

PMID 17450148

Citations 7

Authors

Fulai Jin

Larisa Avramova

Jing Huang

Tony Hazbun

Affiliations

Soon will be listed here.

Abstract

We present here a new two-hybrid smart pool array (SPA) system in which, instead of individual activation domain strains, well-designed activation domain pools are screened in an array format that allows built-in replication and prey-bait deconvolution. Using this method, a Saccharomyces cerevisiae genome SPA increases yeast two-hybrid screening efficiency by an order of magnitude.

Citing Articles

Massively parallel measurement of protein-protein interactions by sequencing using MP3-seq.

Baryshev A, La Fleur A, Groves B, Michel C, Baker D, Ljubetic A Nat Chem Biol. 2024; 20(11):1514-1523.

PMID: 39192093 PMC: 11511666. DOI: 10.1038/s41589-024-01718-x.

Mapping the Protein-Protein Interactome Networks Using Yeast Two-Hybrid Screens.

Rajagopala S Adv Exp Med Biol. 2015; 883:187-214.

PMID: 26621469 PMC: 7120425. DOI: 10.1007/978-3-319-23603-2_11.

Diversity in genetic in vivo methods for protein-protein interaction studies: from the yeast two-hybrid system to the mammalian split-luciferase system.

Stynen B, Tournu H, Tavernier J, Van Dijck P Microbiol Mol Biol Rev. 2012; 76(2):331-82.

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High throughput flow cytometry based yeast two-hybrid array approach for large-scale analysis of protein-protein interactions.

Chen J, Carter M, Edwards B, Cai H, Sklar L Cytometry A. 2011; 81(1):90-8.

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poolMC: smart pooling of mRNA samples in microarray experiments.

Kainkaryam R, Bruex A, Gilbert A, Schiefelbein J, Woolf P BMC Bioinformatics. 2010; 11:299.

PMID: 20525223 PMC: 2900278. DOI: 10.1186/1471-2105-11-299.

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