Autocrine Extracellular Signal-regulated Kinase Activation in Normal Human Keratinocytes is Not Interrupted by Calcium Triggering and is Involved in the Control of Cell Cycle at the Early Stage of Calcium-induced Differentiation

Overview

Journal J Korean Med Sci

Specialty General Medicine

Date 2007 Apr 24

PMID 17449939

Citations 2

Authors

Geon Tae Park

Hyo-Youn Kim

Eun-Kyoung Kim

Jun-Mo Yang

Affiliations

Soon will be listed here.

Abstract

Normal human epidermal keratinocytes (NHEK) respond to the autocrine activated extracellular signal-regulated kinase (ERK) signaling pathway, which contributes to the survival of keratinocytes. However, during the condition of calcium-induced differentiation, how the autocrine ERK signaling is regulated and affected is poorly understood. The purpose of this study was to understand and to obtain clues to the possible function of the autocrine ERK activation during the calcium-induced differentiation of NHEK. We demonstrated that the autocrine activated ERK was not interrupted by calcium triggering and that it was sustained for at least one day after changing the medium. We also found that the autocrine ERK activation was associated with the expression of cyclin D1 and the cell cycle regulation at the early stage of calcium triggering by treating the cells with the mitogen-activated protein kinase inhibitor PD98059. However, the PD98059 treatment did not have a significant influence on the expression of involucrin and loricrin. In addition, we demonstrated that autocrine ERK activation was associated with protein kinase C and p38MAPK signaling. We suggest that the activation of autocrine ERK is not interrupted by calcium triggering and it might participate in cell growth during the early stage of calcium-induced differentiation in NHEK.

Citing Articles

Immunohistochemical Studies of Cytokeratins and Differentiation Markers in Bovine Ocular Squamous Cell Carcinoma.

Vala H, Carvalho T, Pinto C, Pereira M, Mesquita J, Peleteiro M Vet Sci. 2020; 7(2).

PMID: 32485997 PMC: 7355659. DOI: 10.3390/vetsci7020070.

ErbB inhibitors ameliorate behavioral impairments of an animal model for schizophrenia: implication of their dopamine-modulatory actions.

Mizuno M, Sotoyama H, Namba H, Shibuya M, Eda T, Wang R Transl Psychiatry. 2013; 3:e252.

PMID: 23632456 PMC: 3641415. DOI: 10.1038/tp.2013.29.

References

Puri P, Wu Z, Zhang P, Wood L, Bhakta K, Han J . Induction of terminal differentiation by constitutive activation of p38 MAP kinase in human rhabdomyosarcoma cells. Genes Dev. 2000; 14(5):574-84. PMC: 316418. View

Fuchs E, Green H . Changes in keratin gene expression during terminal differentiation of the keratinocyte. Cell. 1980; 19(4):1033-42. DOI: 10.1016/0092-8674(80)90094-x. View

Schmidt M, Goebeler M, Posern G, Feller S, Seitz C, Brocker E . Ras-independent activation of the Raf/MEK/ERK pathway upon calcium-induced differentiation of keratinocytes. J Biol Chem. 2000; 275(52):41011-7. DOI: 10.1074/jbc.M003716200. View

Uchi H, Terao H, Koga T, Furue M . Cytokines and chemokines in the epidermis. J Dermatol Sci. 2001; 24 Suppl 1:S29-38. DOI: 10.1016/s0923-1811(00)00138-9. View

Assoian R, Schwartz M . Coordinate signaling by integrins and receptor tyrosine kinases in the regulation of G1 phase cell-cycle progression. Curr Opin Genet Dev. 2001; 11(1):48-53. DOI: 10.1016/s0959-437x(00)00155-6. View

Coffey Jr R, Derynck R, Wilcox J, Bringman T, Goustin A, Moses H . Production and auto-induction of transforming growth factor-alpha in human keratinocytes. Nature. 1987; 328(6133):817-20. DOI: 10.1038/328817a0. View

Eckert R . Structure, function, and differentiation of the keratinocyte. Physiol Rev. 1989; 69(4):1316-46. DOI: 10.1152/physrev.1989.69.4.1316. View

Fuchs E . Epidermal differentiation. Curr Opin Cell Biol. 1990; 2(6):1028-35. DOI: 10.1016/0955-0674(90)90152-5. View

Hashimoto K, Higashiyama S, Asada H, Hashimura E, Kobayashi T, Sudo K . Heparin-binding epidermal growth factor-like growth factor is an autocrine growth factor for human keratinocytes. J Biol Chem. 1994; 269(31):20060-6. View

10.

Barnard J, Pittelkow M, Dubois R, Cook P, Ramsey G, Bishop P . Auto- and cross-induction within the mammalian epidermal growth factor-related peptide family. J Biol Chem. 1994; 269(36):22817-22. View

11.

Gniadecki R . Stimulation versus inhibition of keratinocyte growth by 1,25-Dihydroxyvitamin D3: dependence on cell culture conditions. J Invest Dermatol. 1996; 106(3):510-6. DOI: 10.1111/1523-1747.ep12343866. View

12.

Cribbs R, Luquette M, Besner G . Acceleration of partial-thickness burn wound healing with topical application of heparin-binding EGF-like growth factor (HB-EGF). J Burn Care Rehabil. 1998; 19(2):95-101. DOI: 10.1097/00004630-199803000-00002. View

13.

Harvat B, Wang A, Seth P, Jetten A . Up-regulation of p27Kip1, p21WAF1/Cip1 and p16Ink4a is associated with, but not sufficient for, induction of squamous differentiation. J Cell Sci. 1998; 111 ( Pt 9):1185-96. DOI: 10.1242/jcs.111.9.1185. View

14.

Zhu A, Haase I, Watt F . Signaling via beta1 integrins and mitogen-activated protein kinase determines human epidermal stem cell fate in vitro. Proc Natl Acad Sci U S A. 1999; 96(12):6728-33. PMC: 21983. DOI: 10.1073/pnas.96.12.6728. View

15.

Kim Y, Seger R, Suresh Babu C, Hwang S, Yoo Y . A positive role of the PI3-K/Akt signaling pathway in PC12 cell differentiation. Mol Cells. 2005; 18(3):353-9. View

16.

Huo X, Zhang J . Annexin1 regulates the erythroid differentiation through ERK signaling pathway. Biochem Biophys Res Commun. 2005; 331(4):1346-52. DOI: 10.1016/j.bbrc.2005.04.049. View

17.

Nowroozi N, Raffioni S, Wang T, Apostol B, Bradshaw R, Thompson L . Sustained ERK1/2 but not STAT1 or 3 activation is required for thanatophoric dysplasia phenotypes in PC12 cells. Hum Mol Genet. 2005; 14(11):1529-38. DOI: 10.1093/hmg/ddi161. View

18.

Chernyavsky A, Arredondo J, Karlsson E, Wessler I, Grando S . The Ras/Raf-1/MEK1/ERK signaling pathway coupled to integrin expression mediates cholinergic regulation of keratinocyte directional migration. J Biol Chem. 2005; 280(47):39220-8. DOI: 10.1074/jbc.M504407200. View

19.

Haase I, Hobbs R, Romero M, Broad S, Watt F . A role for mitogen-activated protein kinase activation by integrins in the pathogenesis of psoriasis. J Clin Invest. 2001; 108(4):527-36. PMC: 209397. DOI: 10.1172/JCI12153. View

20.

Iordanov M, Choi R, Ryabinina O, Bright R, Magun B . The UV (Ribotoxic) stress response of human keratinocytes involves the unexpected uncoupling of the Ras-extracellular signal-regulated kinase signaling cascade from the activated epidermal growth factor receptor. Mol Cell Biol. 2002; 22(15):5380-94. PMC: 133934. DOI: 10.1128/MCB.22.15.5380-5394.2002. View