Rapid Downregulation of NCX and PMCA in Hippocampal Neurons Following H2O2 Oxidative Stress

Overview

Journal Ann N Y Acad Sci

Specialty Science

Date 2007 Apr 21

PMID 17446483

Citations 17

Authors

Sertac N Kip

Emanuel E Strehler

Affiliations

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Abstract

Na+/Ca2+ exchangers (NCXs) and plasma membrane Ca2+ pumps (PMCAs) are crucial for intracellular Ca2+ homeostasis and Ca2+ signaling. Elevated [Ca2+]i is a hallmark of neurodegenerative disease and stroke. Here we studied the short-term effect of oxidative stress on the plasma membrane Ca2+ extrusion systems in hippocampal neurons (HN) and found that after 2-3 h exposure to 300 microM H2O2, all NCXs and PMCAs were significantly downregulated at the RNA (NCX) and protein (PMCA) level. Rapid internalization and aggregation of the PMCA was also observed. Our data show that the plasma membrane calcium extrusion systems are sensitive early targets of neurotoxic oxidative stress.

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References

Coyle J, Puttfarcken P . Oxidative stress, glutamate, and neurodegenerative disorders. Science. 1993; 262(5134):689-95. DOI: 10.1126/science.7901908. View

Choi D . Calcium: still center-stage in hypoxic-ischemic neuronal death. Trends Neurosci. 1995; 18(2):58-60. View

Yu L, Colvin R . Regional differences in expression of transcripts for Na+/Ca2+ exchanger isoforms in rat brain. Brain Res Mol Brain Res. 1997; 50(1-2):285-92. DOI: 10.1016/s0169-328x(97)00202-7. View

Kip S, Gray N, Burette A, Canbay A, Weinberg R, Strehler E . Changes in the expression of plasma membrane calcium extrusion systems during the maturation of hippocampal neurons. Hippocampus. 2005; 16(1):20-34. PMC: 3873839. DOI: 10.1002/hipo.20129. View

Papa M, Canitano A, Boscia F, Castaldo P, Sellitti S, Porzig H . Differential expression of the Na+-Ca2+ exchanger transcripts and proteins in rat brain regions. J Comp Neurol. 2003; 461(1):31-48. DOI: 10.1002/cne.10665. View