The Temperature-sensitive Role of Cryptococcus Neoformans ROM2 in Cell Morphogenesis

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2007 Apr 12

PMID 17426816

Citations 10

Authors

Beth Burgwyn Fuchs

Robin J Tang

Eleftherios Mylonakis

Affiliations

Soon will be listed here.

Abstract

ROM2 is associated with Cryptococcus neoformans virulence. We examined additional roles of ROM2 in C. neoformans and found that ROM2 plays a role in several cell functions specifically at high temperature conditions. Morphologically rom2 mutant cells demonstrated a "tear"-like shape and clustered together. A sub-population of cells had a hyperelongated phenotype at restrictive growth conditions. Altered morphology was associated with defects in actin that was concentrated at the cell periphery and with abnormalities in microtubule organization. Interestingly, the ROM2 associated defects in cell morphology, location of nuclei, and actin and microtubule organization were not observed in cells grown at temperatures below 37 degrees C. These results indicate that in C. neoformans, ROM2 is important at restrictive temperature conditions and is involved in several cell maintenance functions.

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