Viruses and Virus-like Particles Detected During Examination of Feces from Calves and Piglets with Diarrhea
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A total of 763 fecal or intestinal samples from diarrheic calves and piglets were examined for viral content by immunofluorescence, electron microscopy or cell culture. Routine fluorescent antibody and cultural tests detected rotavirus (n=126), coronavirus (n=80) and bovine viral diarrhea virus (n=13). Electron microscopy detected rotaviruses (n=24) and coronaviruses (n=17) not identified by standard fluorescent antibody tests. Other viruses detected by electron microscopy included Breda virus-like particles (n=49), astroviruses (n=1), caliciviruses (n=1), rhabdoviruses (n=1), parvoviruses (n=2), enteroviruses (n=3), togavirus-like particles (n=2), and "chained" particles (n=5). Mixtures of several of the viruses were detected in a number of fecal samples.The survey emphasized the value of electron microscopy as a broad-spectrum diagnostic tool.
Classification, replication, and transcription of .
Liao Y, Wang H, Liao H, Sun Y, Tan L, Song C Front Microbiol. 2024; 14:1291761.
PMID: 38328580 PMC: 10847374. DOI: 10.3389/fmicb.2023.1291761.
Recent Progress in Torovirus Molecular Biology.
Ujike M, Taguchi F Viruses. 2021; 13(3).
PMID: 33800523 PMC: 7998386. DOI: 10.3390/v13030435.
Xu S, Zhou J, Chen Y, Tong X, Wang Z, Guo J J Virol. 2020; 94(20).
PMID: 32727876 PMC: 7527039. DOI: 10.1128/JVI.01282-20.
Porcine Torovirus (PToV)-A Brief Review of Etiology, Diagnostic Assays and Current Epidemiology.
Hu Z, Yang Y, Xu L, Wang B, Qin P, Huang Y Front Vet Sci. 2019; 6:120.
PMID: 31058174 PMC: 6482245. DOI: 10.3389/fvets.2019.00120.
A Naturally Occurring Recombinant Enterovirus Expresses a Torovirus Deubiquitinase.
Shang P, Misra S, Hause B, Fang Y J Virol. 2017; 91(14).
PMID: 28490584 PMC: 5487566. DOI: 10.1128/JVI.00450-17.