Human and Human-yeast Chimeric U6 SnRNA Genes Identify Structural Elements Required for Expression in Yeast

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1992 Feb 11

PMID 1741282

Citations 5

Authors

R Bordonne

C Guthrie

Affiliations

Soon will be listed here.

Abstract

U6 is the most highly conserved spliceosomal snRNA. Previous mutational studies have shown that the majority of essential residues in U6 are located in a region of 35 nucleotides encompassing a conserved hexanucleotide and stem I and stem II of the U4-interaction domain. Although the yeast and human U6 RNAs are 80% identical in this region, the human U6 gene cannot functionally replace the yeast gene in vivo. The human gene is not transcribed when placed in the context of yeast flanking sequences. Transcription of the human gene, but not its function, can be stimulated by the introduction of an A block promoter element in the U6 coding region. Using a set of human-yeast chimeras, we show that the 5' domain and the 3' terminal region of the human U6 gene can each functionally replace the corresponding yeast domains. However, a combination of both domains in a single molecule is lethal. The basis of the inability of the human U6 snRNA to function in yeast cells is discussed.

Citing Articles

Transcription-dependent enrichment of the yeast FACT complex influences nucleosome dynamics on the RNA polymerase III-transcribed genes.

Shukla A, Bhalla P, Potdar P, Jampala P, Bhargava P RNA. 2020; .

PMID: 33277439 PMC: 7901838. DOI: 10.1261/rna.077974.120.

Termination of pre-mRNA splicing requires that the ATPase and RNA unwindase Prp43p acts on the catalytic snRNA U6.

Toroney R, Nielsen K, Staley J Genes Dev. 2019; 33(21-22):1555-1574.

PMID: 31558568 PMC: 6824469. DOI: 10.1101/gad.328294.119.

The 5' and 3' domains of yeast U6 snRNA: Lsm proteins facilitate binding of Prp24 protein to the U6 telestem region.

Ryan D, Stevens S, Abelson J RNA. 2002; 8(8):1011-33.

PMID: 12212846 PMC: 1370313. DOI: 10.1017/s1355838202026092.

Architecture of a yeast U6 RNA gene promoter.

Eschenlauer J, Kaiser M, Gerlach V, Brow D Mol Cell Biol. 1993; 13(5):3015-26.

PMID: 8474459 PMC: 359694. DOI: 10.1128/mcb.13.5.3015-3026.1993.

Transcription of a variant human U6 small nuclear RNA gene is controlled by a novel, internal RNA polymerase III promoter.

Tichelaar J, Knerer B, Vrabel A, Wieben E Mol Cell Biol. 1994; 14(8):5450-7.

PMID: 8035822 PMC: 359064. DOI: 10.1128/mcb.14.8.5450-5457.1994.

References

Wu J, Manley J . Base pairing between U2 and U6 snRNAs is necessary for splicing of a mammalian pre-mRNA. Nature. 1991; 352(6338):818-21. DOI: 10.1038/352818a0. View

Riedel N, Wolin S, Guthrie C . A subset of yeast snRNA's contains functional binding sites for the highly conserved Sm antigen. Science. 1987; 235(4786):328-31. DOI: 10.1126/science.2948278. View

Shannon K, Guthrie C . Suppressors of a U4 snRNA mutation define a novel U6 snRNP protein with RNA-binding motifs. Genes Dev. 1991; 5(5):773-85. DOI: 10.1101/gad.5.5.773. View

Datta B, Weiner A . Genetic evidence for base pairing between U2 and U6 snRNA in mammalian mRNA splicing. Nature. 1991; 352(6338):821-4. DOI: 10.1038/352821a0. View

Siliciano P, Kivens W, Guthrie C . More than half of yeast U1 snRNA is dispensable for growth. Nucleic Acids Res. 1991; 19(23):6367-72. PMC: 329179. DOI: 10.1093/nar/19.23.6367. View

Miraglia L, Seiwert S, Igel A, Ares Jr M . Limited functional equivalence of phylogenetic variation in small nuclear RNA: yeast U2 RNA with altered branchpoint complementarity inhibits splicing and produces a dominant lethal phenotype. Proc Natl Acad Sci U S A. 1991; 88(16):7061-5. PMC: 52233. DOI: 10.1073/pnas.88.16.7061. View

Guthrie C . Messenger RNA splicing in yeast: clues to why the spliceosome is a ribonucleoprotein. Science. 1991; 253(5016):157-63. DOI: 10.1126/science.1853200. View

Tani T, Ohshima Y . mRNA-type introns in U6 small nuclear RNA genes: implications for the catalysis in pre-mRNA splicing. Genes Dev. 1991; 5(6):1022-31. DOI: 10.1101/gad.5.6.1022. View

Ruby S, Abelson J . Pre-mRNA splicing in yeast. Trends Genet. 1991; 7(3):79-85. DOI: 10.1016/0168-9525(91)90276-V. View

10.

Hausner T, Giglio L, Weiner A . Evidence for base-pairing between mammalian U2 and U6 small nuclear ribonucleoprotein particles. Genes Dev. 1990; 4(12A):2146-56. DOI: 10.1101/gad.4.12a.2146. View

11.

Madhani H, Bordonne R, Guthrie C . Multiple roles for U6 snRNA in the splicing pathway. Genes Dev. 1990; 4(12B):2264-77. DOI: 10.1101/gad.4.12b.2264. View

12.

Liao X, Kretzner L, Seraphin B, Rosbash M . Universally conserved and yeast-specific U1 snRNA sequences are important but not essential for U1 snRNP function. Genes Dev. 1990; 4(10):1766-74. DOI: 10.1101/gad.4.10.1766. View

13.

Bordonne R, Banroques J, Abelson J, Guthrie C . Domains of yeast U4 spliceosomal RNA required for PRP4 protein binding, snRNP-snRNP interactions, and pre-mRNA splicing in vivo. Genes Dev. 1990; 4(7):1185-96. DOI: 10.1101/gad.4.7.1185. View

14.

Vankan P, McGuigan C, Mattaj I . Domains of U4 and U6 snRNAs required for snRNP assembly and splicing complementation in Xenopus oocytes. EMBO J. 1990; 9(10):3397-404. PMC: 552079. DOI: 10.1002/j.1460-2075.1990.tb07541.x. View

15.

Fabrizio P, Abelson J . Two domains of yeast U6 small nuclear RNA required for both steps of nuclear precursor messenger RNA splicing. Science. 1990; 250(4979):404-9. DOI: 10.1126/science.2145630. View

16.

Brow D, Guthrie C . Transcription of a yeast U6 snRNA gene requires a polymerase III promoter element in a novel position. Genes Dev. 1990; 4(8):1345-56. DOI: 10.1101/gad.4.8.1345. View

17.

Kunkel G, Pederson T . Transcription of a human U6 small nuclear RNA gene in vivo withstands deletion of intragenic sequences but not of an upstream TATATA box. Nucleic Acids Res. 1989; 17(18):7371-9. PMC: 334816. DOI: 10.1093/nar/17.18.7371. View

18.

Murphy S, Moorefield B, Pieler T . Common mechanisms of promoter recognition by RNA polymerases II and III. Trends Genet. 1989; 5(4):122-6. DOI: 10.1016/0168-9525(89)90043-7. View

19.

Carbon P, Murgo S, Ebel J, Krol A, Tebb G, Mattaj L . A common octamer motif binding protein is involved in the transcription of U6 snRNA by RNA polymerase III and U2 snRNA by RNA polymerase II. Cell. 1987; 51(1):71-9. DOI: 10.1016/0092-8674(87)90011-0. View

20.

Pan Z, Prives C . Assembly of functional U1 and U2 human-amphibian hybrid snRNPs in Xenopus laevis oocytes. Science. 1988; 241(4871):1328-31. DOI: 10.1126/science.2970672. View