Development of an Affinity Silica Monolith Containing Alpha1-acid Glycoprotein for Chiral Separations

Overview

Journal J Chromatogr A

Publisher Elsevier

Specialty Chemistry

Date 2007 Apr 6

PMID 17408678

Citations 21

Authors

Rangan Mallik

Hai Xuan

David S Hage

Affiliations

Soon will be listed here.

Abstract

An affinity monolith based on silica and containing immobilized alpha(1)-acid glycoprotein (AGP) was developed and evaluated in terms of its binding, efficiency and selectivity in chiral separations. The results were compared with data obtained for the same protein when used as a chiral stationary phase with HPLC-grade silica particles or monoliths based on a copolymer of glycidyl methacrylate (GMA) and ethylene dimethacrylate (EDMA). The surface coverage of AGP in the silica monolith was 18% higher than that obtained with silica particles and 61% higher than that measured for a GMA/EDMA monolith. The higher surface area of the silica monolith gave materials that contained 1.5- to 3.6-times more immobilized protein per unit volume when compared to silica particles or a GMA/EDMA monolith. The retention, efficiency and resolving power of the AGP silica monolith were evaluated by injecting two chiral analytes onto this column (i.e., R/S-warfarin and R/S-propranolol). In each case, the AGP silica monolith gave higher retention plus better resolution and efficiency than AGP columns containing silica particles or a GMA/EDMA monolith. The AGP silica monolith also gave lower back pressures and separation impedances than these other materials. It was concluded that silica monoliths can be valuable alternatives to silica particles or GMA/EDMA monoliths when used with AGP as a chiral stationary phase.

Citing Articles

Chiral Monolithic Silica-Based HPLC Columns for Enantiomeric Separation and Determination: Functionalization of Chiral Selector and Recognition of Selector-Selectand Interaction.

Asmari M, Wang X, Casado N, Piponski M, Kovalenko S, Logoyda L Molecules. 2021; 26(17).

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Affinity monolith chromatography: A review of general principles and recent developments.

Poddar S, Sharmeen S, Hage D Electrophoresis. 2021; 42(24):2577-2598.

PMID: 34293192 PMC: 9536602. DOI: 10.1002/elps.202100163.

Rapid Purification of Immunoglobulin G Using a Protein A-immobilized Monolithic Spin Column with Hydrophilic Polymers.

Ota S, Yui Y, Sato T, Yoshimoto N, Yamamoto S Anal Sci. 2020; 37(7):985-990.

PMID: 33281136 DOI: 10.2116/analsci.20P378.

High performance affinity chromatography and related separation methods for the analysis of biological and pharmaceutical agents.

Zhang C, Rodriguez E, Bi C, Zheng X, Suresh D, Suh K Analyst. 2017; 143(2):374-391.

PMID: 29200216 PMC: 5768458. DOI: 10.1039/c7an01469d.

Affinity monolith chromatography: A review of general principles and applications.

Li Z, Rodriguez E, Azaria S, Pekarek A, Hage D Electrophoresis. 2017; 38(22-23):2837-2850.

PMID: 28474739 PMC: 5671914. DOI: 10.1002/elps.201700101.

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