Bovine Apolipoprotein B-100 is a Dominant Immunogen in Therapeutic Cell Populations Cultured in Fetal Calf Serum in Mice and Humans

Overview

Journal Blood

Publisher Elsevier

Specialty Hematology

Date 2007 Mar 31

PMID 17395779

Citations 22

Authors

Norihisa Sakamoto

Kazuhide Tsuji

Linda M Muul

Ann M Lawler

Emanuel F Petricoin

Fabio Candotti

Julia A Metcalf

Jorge A Tavel

H Clifford Lane

Walter J Urba

Bernard A Fox

Ajit Varki

Joan K Lunney

Amy S Rosenberg

Affiliations

Soon will be listed here.

Abstract

Recent studies have demonstrated that cell populations intended for therapeutic purposes that are cultured in heterologous animal products can acquire xenoantigens, potentially limiting their utility. In investigations of the immune response to murine embryonic stem cells, we found that a strong antibody response was generated after the second infusion. Both polyclonal and monoclonal antibody responses, derived from immunized mice, were found to be specific for bovine apolipoprotein B-100, which binds to abundant low-density lipoprotein receptors on the cell surface and is internalized. Here we show that in the majority of patients administered 3 different types of cell-based therapies using cells grown in fetal calf serum-containing media, an antibody response to bovine apolipoprotein B-100 develops after the second infusion and is the dominant specificity. The known and potential clinical effects of such antibodies are discussed.

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