The Role of Pannexin 1 Hemichannels in ATP Release and Cell-cell Communication in Mouse Taste Buds

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2007 Mar 29

PMID 17389364

Citations 262

Authors

Yi-Jen Huang

Yutaka Maruyama

Gennady Dvoryanchikov

Elizabeth Pereira

Nirupa Chaudhari

Stephen D Roper

Affiliations

Soon will be listed here.

Abstract

ATP has been shown to be a taste bud afferent transmitter, but the cells responsible for, and the mechanism of, its release have not been identified. Using CHO cells expressing high-affinity neurotransmitter receptors as biosensors, we show that gustatory stimuli cause receptor cells to secrete ATP through pannexin 1 hemichannels in mouse taste buds. ATP further stimulates other taste cells to release a second transmitter, serotonin. These results provide a mechanism to link intracellular Ca(2+) release during taste transduction to secretion of afferent transmitter, ATP, from receptor cells. They also indicate a route for cell-cell communication and signal processing within the taste bud.

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