Inhibition of DNA Synthesis at the Hemimethylated PBR322 Origin of Replication by a Cell Membrane Fraction

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1992 Jan 11

PMID 1738587

Citations 2

Authors

A Malki

R Kern

M Kohiyama

P Hughes

Affiliations

Soon will be listed here.

Abstract

The replication of both ColE1-type plasmids and plasmids bearing the origin of replication of the Escherichia coli chromosome (oriC) has been shown to be inhibited by hemimethylation of adenine residues within GATC sequences. In the case of oriC plasmids, this inhibition was previously shown to be mediated by the specific affinity of the hemimethylated origin DNA for an outer cell membrane fraction. Here, we suggest that a similar mechanism is operating in the case of the ColE1-like plasmid pBR322 as (i) a hemimethylated DNA fragment carrying the promoter for the RNA which primes DNA synthesis (RNAII) is specifically bound by the same membrane fraction and, (ii) the addition of the membrane fraction to a soluble assay of pBR322 replication results in preferential inhibition of initiation on the hemimethylated template. We suggest that membrane sequestration of hemimethylated origin DNA and/or associated replication genes following replication may be a common element restricting DNA replication to precise moments in the cell cycle.

Citing Articles

mioC transcription, initiation of replication, and the eclipse in Escherichia coli.

Bogan J, Helmstetter C J Bacteriol. 1996; 178(11):3201-6.

PMID: 8655499 PMC: 178071. DOI: 10.1128/jb.178.11.3201-3206.1996.

Densely methylated DNA islands in mammalian chromosomal replication origins.

Tasheva E, Roufa D Mol Cell Biol. 1994; 14(9):5636-44.

PMID: 8065300 PMC: 359088. DOI: 10.1128/mcb.14.9.5636-5644.1994.

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