Photo-physical Properties of Ca2+-indicator Dyes Suitable for Two-photon Fluorescence-lifetime Recordings

Overview

Journal J Microsc

Specialty Radiology

Date 2007 Mar 21

PMID 17371443

Citations 23

Authors

Christian D Wilms

Jens Eilers

Affiliations

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Abstract

We analyzed the suitability of various Ca2+-indicator dyes for quantitative two-photon fluorescence-lifetime imaging. Although fura-2, fluo-3, BTC and calcein did not show useful Ca2+-dependent lifetime changes, calcium orange, calcium green-1, oregon green-2 and -5N, as well as magnesium green allowed to quantify the Ca2+-free and Ca2+-bound dye fractions by a double-exponential lifetime analysis. For the latter dyes, we derived calibration formalisms that correct for lifetime distortions by dye impurities and Ca2+-dependent extinction coefficients.

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