Panmicrobial Oligonucleotide Array for Diagnosis of Infectious Diseases

Overview

Journal Emerg Infect Dis

Specialty Infectious Diseases

Date 2007 Mar 21

PMID 17370518

Citations 151

Authors

Gustavo Palacios

Phenix-Lan Quan

Omar J Jabado

Sean Conlan

David L Hirschberg

Yang Liu

Junhui Zhai

Neil Renwick

Jeffrey Hui

Hedi Hegyi

Allen Grolla

James E Strong

Jonathan S Towner

Thomas W Geisbert

Peter B Jahrling

Cornelia Buchen-Osmond

Heinz Ellerbrok

Maria Paz Sanchez-Seco

Yves Lussier

Pierre Formenty

M Stuart T Nichol

Heinz Feldmann

Thomas Briese

W Ian Lipkin

Affiliations

Soon will be listed here.

Abstract

To facilitate rapid, unbiased, differential diagnosis of infectious diseases, we designed GreeneChipPm, a panmicrobial microarray comprising 29,455 sixty-mer oligonucleotide probes for vertebrate viruses, bacteria, fungi, and parasites. Methods for nucleic acid preparation, random primed PCR amplification, and labeling were optimized to allow the sensitivity required for application with nucleic acid extracted from clinical materials and cultured isolates. Analysis of nasopharyngeal aspirates, blood, urine, and tissue from persons with various infectious diseases confirmed the presence of viruses and bacteria identified by other methods, and implicated Plasmodium falciparum in an unexplained fatal case of hemorrhagic feverlike disease during the Marburg hemorrhagic fever outbreak in Angola in 2004-2005.

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