Reduced Expression of AP27 Protein, the Product of a Growth Factor-repressible Gene, is Associated with Diminished Adipocyte Differentiation

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1992 Feb 1

PMID 1736287

Citations 6

Authors

H M Wenz

L Hinck

P Cannon

M Navre

G M Ringold

Affiliations

Soon will be listed here.

Abstract

We have recently characterized an adipocyte cDNA (clone 5) that is enhanced in expression by environmental and hormonal conditions favoring adipogenic differentiation. Moreover, certain agents including fibroblast growth factor and phorbol 12-myristate 13-acetate (but not epidermal growth factor) markedly inhibit clone 5 gene expression and prevent TA1 cell differentiation. These results led us to propose that a threshold level of the clone 5 gene product (AP27 protein) is required for triggering adipocyte differentiation. We have constructed vectors that direct the synthesis of clone 5 antisense RNA to reduce the levels of AP27 in adipogenic cell lines TA1 and 3T3-L1. We show here that when these cells express clone 5 antisense RNA, they fail to undergo morphological differentiation, whereas adipogenesis is unaffected in cells expressing antisense beta-actin or ferritin heavy-chain RNA. We further show that cells expressing clone 5 antisense RNA (but not the other antisense RNAs) are unable to induce the expression of characteristic "adipocyte-specific" mRNAs. The level of inhibition of differentiation by clone 5 antisense RNA correlates with decreased levels of AP27 protein. These results provide strong evidence that expression of AP27 is linked to adipogenic differentiation and that AP27 may be a component of an as-yet-uncharacterized signal-transduction pathway required for the triggering of adipocyte differentiation.

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References

Izant J, Weintraub H . Constitutive and conditional suppression of exogenous and endogenous genes by anti-sense RNA. Science. 1985; 229(4711):345-52. DOI: 10.1126/science.2990048. View

Felgner P, Gadek T, Holm M, Roman R, Chan H, Wenz M . Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure. Proc Natl Acad Sci U S A. 1987; 84(21):7413-7. PMC: 299306. DOI: 10.1073/pnas.84.21.7413. View

Hunt C, Ro J, Dobson D, Min H, Spiegelman B . Adipocyte P2 gene: developmental expression and homology of 5'-flanking sequences among fat cell-specific genes. Proc Natl Acad Sci U S A. 1986; 83(11):3786-90. PMC: 323608. DOI: 10.1073/pnas.83.11.3786. View

Rhodes S, Konieczny S . Identification of MRF4: a new member of the muscle regulatory factor gene family. Genes Dev. 1989; 3(12B):2050-61. DOI: 10.1101/gad.3.12b.2050. View

Massague J, Cheifetz S, Endo T, Nadal-Ginard B . Type beta transforming growth factor is an inhibitor of myogenic differentiation. Proc Natl Acad Sci U S A. 1986; 83(21):8206-10. PMC: 386896. DOI: 10.1073/pnas.83.21.8206. View

Korman A, Frantz J, Strominger J, Mulligan R . Expression of human class II major histocompatibility complex antigens using retrovirus vectors. Proc Natl Acad Sci U S A. 1987; 84(8):2150-4. PMC: 304606. DOI: 10.1073/pnas.84.8.2150. View

Chen S, Teicher L, Kazim D, Pollack R, Wise L . Commitment of mouse fibroblasts to adipocyte differentiation by DNA transfection. Science. 1989; 244(4904):582-5. DOI: 10.1126/science.2470149. View

Navre M, Ringold G . A growth factor-repressible gene associated with protein kinase C-mediated inhibition of adipocyte differentiation. J Cell Biol. 1988; 107(1):279-86. PMC: 2115191. DOI: 10.1083/jcb.107.1.279. View

Kirchgessner T, LeBoeuf R, Langner C, Zollman S, Chang C, Taylor B . Genetic and developmental regulation of the lipoprotein lipase gene: loci both distal and proximal to the lipoprotein lipase structural gene control enzyme expression. J Biol Chem. 1989; 264(3):1473-82. View

10.

Wright W, Sassoon D, Lin V . Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD. Cell. 1989; 56(4):607-17. DOI: 10.1016/0092-8674(89)90583-7. View

11.

Agarwal A, Monder C, Eckstein B, White P . Cloning and expression of rat cDNA encoding corticosteroid 11 beta-dehydrogenase. J Biol Chem. 1989; 264(32):18939-43. View

12.

Braun T, Bober E, Tannich E, Arnold H . A novel human muscle factor related to but distinct from MyoD1 induces myogenic conversion in 10T1/2 fibroblasts. EMBO J. 1989; 8(3):701-9. PMC: 400865. DOI: 10.1002/j.1460-2075.1989.tb03429.x. View

13.

Melton D, Krieg P, Rebagliati M, Maniatis T, Zinn K, Green M . Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 1984; 12(18):7035-56. PMC: 320141. DOI: 10.1093/nar/12.18.7035. View

14.

Chapman A, Knight D, Dieckmann B, Ringold G . Analysis of gene expression during differentiation of adipogenic cells in culture and hormonal control of the developmental program. J Biol Chem. 1984; 259(24):15548-55. View

15.

FLORINI J, Roberts A, Ewton D, Falen S, Flanders K, Sporn M . Transforming growth factor-beta. A very potent inhibitor of myoblast differentiation, identical to the differentiation inhibitor secreted by Buffalo rat liver cells. J Biol Chem. 1986; 261(35):16509-13. View

16.

Davis R, Weintraub H, Lassar A . Expression of a single transfected cDNA converts fibroblasts to myoblasts. Cell. 1987; 51(6):987-1000. DOI: 10.1016/0092-8674(87)90585-x. View

17.

Ringold G, Chapman A, Knight D . Glucocorticoid control of developmentally regulated adipose genes. J Steroid Biochem. 1986; 24(1):69-75. DOI: 10.1016/0022-4731(86)90034-8. View

18.

Chapman A, Knight D, Ringold G . Glucocorticoid regulation of adipocyte differentiation: hormonal triggering of the developmental program and induction of a differentiation-dependent gene. J Cell Biol. 1985; 101(4):1227-35. PMC: 2113934. DOI: 10.1083/jcb.101.4.1227. View

19.

Cook K, Hunt C, Spiegelman B . Developmentally regulated mRNAs in 3T3-adipocytes: analysis of transcriptional control. J Cell Biol. 1985; 100(2):514-20. PMC: 2113435. DOI: 10.1083/jcb.100.2.514. View

20.

Baker M . A common ancestor for human placental 17 beta-hydroxysteroid dehydrogenase, Streptomyces coelicolor actIII protein, and Drosophila melanogaster alcohol dehydrogenase. FASEB J. 1990; 4(2):222-6. DOI: 10.1096/fasebj.4.2.2153594. View